CD45R Antibody (RA3-6B2): sc-19597

CD45R Antibody (RA3-6B2) is a rat monoclonal IgG2a light chain antibody engineered to detect CD45, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC), in mouse, rat, and human samples through western blotting (WB), immunoprecipitation (IP), PAR assays, and flow cytometry (FCM). Anti-CD45 antibody (2D-1) targets the extracellular domain of human CD45, ensuring reliable recognition across multiple species. CD45 is a vital transmembrane glycoprotein expressed on all nucleated hematopoietic cells, where it functions as a protein tyrosine phosphatase essential for regulating immune cell signaling and activation. Structurally, CD45 consists of an extracellular region with multiple isoforms generated through alternative splicing of exons, a single transmembrane domain, and a conserved intracellular phosphatase domain. Post-translational modifications, particularly glycosylation, are crucial for CD45′s function as they influence its stability, localization, and interactions with other proteins, thereby modulating T and B cell receptor signaling pathways. These modifications affect how CD45 dephosphorylates Src and Syk family kinases, such as Lck, Fyn, and ZAP-70, which are vital for initiating downstream signaling cascades that lead to T cell activation, proliferation, differentiation, and B cell development. The structural organization and glycosylation patterns of CD45 enable fine-tuned regulation of immune responses, allowing tailored interactions depending on the cell type and activation state. Dysregulation or alterations in CD45′s structure and post-translational modifications have been linked to various immune disorders, including autoimmune diseases and leukemias, highlighting its importance in maintaining immune homeostasis and its potential as a therapeutic target. CD45 monoclonal antibody (2D-1) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing versatile options for a wide range of experimental applications. Anti-CD45 antibody (2D-1) enables researchers to effectively study the expression, isoform distribution, and post-translational modifications of CD45, thereby advancing our understanding of immune cell biology and related disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD45R Antibody (RA3-6B2) References:

1. Sialic acid-dependent epitopes of CD45 molecules of restricted cellular expression.  |  Bazil, V., et al. 1989. Immunogenetics. 29: 202-5. PMID: 2466778
2. The leukocyte common antigen family.  |  Thomas, ML. 1989. Annu Rev Immunol. 7: 339-69. PMID: 2523715
3. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102.  |  Bazil, V., et al. 1989. Folia Biol (Praha). 35: 289-97. PMID: 2534480
4. Molecular and antigenic heterogeneity of the rat leukocyte-common antigen from thymocytes and T and B lymphocytes.  |  Woollett, GR., et al. 1985. Eur J Immunol. 15: 168-73. PMID: 2578966
5. Differential usage of three exons generates at least five different mRNAs encoding human leukocyte common antigens.  |  Streuli, M., et al. 1987. J Exp Med. 166: 1548-66. PMID: 2824653
6. CD45 in human physiology and clinical medicine.  |  Rheinländer, A., et al. 2018. Immunol Lett. 196: 22-32. PMID: 29366662
7. Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections.  |  Poppema, S., et al. 1987. Am J Pathol. 127: 418-29. PMID: 3296769
8. The demonstration of B-cell, T-cell and myeloid antigens in paraffin sections.  |  West, KP., et al. 1986. J Pathol. 150: 89-101. PMID: 3540244
9. New marker of B lymphocytes, MB2: comparison with other lymphocyte subset markers active in conventionally processed tissue sections.  |  Hall, PA., et al. 1987. J Clin Pathol. 40: 151-6. PMID: 3546393
10. Epitope base editing CD45 in hematopoietic cells enables universal blood cancer immune therapy.  |  Wellhausen, N., et al. 2023. Sci Transl Med. 15: eadi1145. PMID: 37651540
11. The role of CD45 and CD45-associated molecules in T cell activation.  |  Altin, JG. and Sloan, EK. 1997. Immunol Cell Biol. 75: 430-45. PMID: 9429890

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD45R Antibody (RA3-6B2) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-CD45R Antibody (RA3-6B2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD45R Antibody (RA3-6B2) Specifications

Alternate Product Name: CD45

Product Clone Name: RA3-6B2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: CD45R

Epitope Species: mouse

Product Reactivity/Detection: CD45R

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for CD45R Antibody (RA3-6B2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, APC-Cy7: Allophycocyanin with Cyanin-7, FITC: Fluoroscein, HRP: Horseradish Peroxidase, L: Azide-Free, PE: Phycoerythrin

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.