CD45 Antibody (2D-1): sc-1187

CD45 Antibody (2D-1) is a mouse monoclonal IgG1 kappa light chain antibody engineered to detect CD45, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC), in mouse, rat, and human samples using western blotting (WB), immunoprecipitation (IP), PAR assays, and flow cytometry (FCM). CD45 monoclonal antibody (2D-1) targets the extracellular domain of human CD45. CD45 is a vital transmembrane glycoprotein prominently expressed on the surface of all hematopoietic cells, where CD45 plays a crucial role in regulating immune cell signaling and activation. Located on the cell membrane, CD45 acts as a key regulator of T and B cell receptor signaling by dephosphorylating specific tyrosine residues on Src and Syk family kinases, such as Lck, Fyn, and ZAP-70. This modulation is essential for proper immune responses, including T cell activation, proliferation, and differentiation, as well as B cell development and antibody production. The extracellular domain of CD45 undergoes alternative splicing, resulting in multiple isoforms that provide fine-tuned regulation of immune functions tailored to different cell types and activation states. The precise localization of CD45 on the cell surface facilitates CD45 interaction with other membrane-bound receptors and signaling molecules, making CD45 indispensable for maintaining immune homeostasis and responding to pathogens. Dysregulation or altered expression of CD45 has been implicated in various immune disorders, including autoimmune diseases and leukemias, highlighting CD45 significance in both normal immune function and disease pathogenesis. Anti-CD45 antibody (2D-1) is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and Alexa Fluor® conjugates, providing versatile options for a wide range of experimental applications. By utilizing CD45 monoclonal antibody (2D-1), researchers can effectively study the expression, isoform distribution, and functional dynamics of CD45, thereby advancing our understanding of immune cell biology and related disease mechanisms.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CD45 Antibody (2D-1) References:

1. The CD45 tyrosine phosphatase: a positive and negative regulator of immune cell function.  |  Alexander, DR. 2000. Semin Immunol. 12: 349-59. PMID: 10995582
2. New insights into the transmembrane protein tyrosine phosphatase CD45.  |  Sasaki, T., et al. 2001. Int J Biochem Cell Biol. 33: 1041-6. PMID: 11551820
3. A cellular model for myeloma cell growth and maturation based on an intraclonal CD45 hierarchy.  |  Bataille, R., et al. 2003. Immunol Rev. 194: 105-11. PMID: 12846811
4. A cell death pathway induced by antibody-mediated cross-linking of CD45 on lymphocytes.  |  Steff, AM., et al. 2003. Crit Rev Immunol. 23: 421-40. PMID: 15030310
5. Viral interference with functions of the cellular receptor tyrosine phosphatase CD45.  |  Thiel, N., et al. 2015. Viruses. 7: 1540-57. PMID: 25807057
6. Partial endothelial-to-mesenchymal transition mediated by HIF-induced CD45 in neointima formation upon carotid artery ligation.  |  Yamashiro, Y., et al. 2023. Cardiovasc Res. 119: 1606-1618. PMID: 36537041
7. Expression of CD45 in non-hematopoietic cells: implications in regenerative medicine and disease management.  |  Das, M., et al. 2024. Regen Med. 19: 407-419. PMID: 39058408
8. CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development.  |  Trowbridge, IS. and Thomas, ML. 1994. Annu Rev Immunol. 12: 85-116. PMID: 8011300
9. The leukocyte common antigen, CD45 and other protein tyrosine phosphatases in hematopoietic cells.  |  Woodford-Thomas, T. and Thomas, ML. 1993. Semin Cell Biol. 4: 409-18. PMID: 8305680
10. CD45-associated protein is a lymphocyte-specific membrane protein expressed in two distinct forms.  |  Kitamura, K., et al. 1997. Eur J Immunol. 27: 383-8. PMID: 9045908

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD45 Antibody (2D-1) has 22 citations in a variety of scientific publications.

Primary antibodies like Anti-CD45 Antibody (2D-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD45 Antibody (2D-1) Specifications

Alternate Product Name: PTPRC

Product Clone Name: 2D-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD45

Epitope Species: human

Product Reactivity/Detection: CD45, CD45RO, CD45A, CD45B and all forms of LCA

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 22 citations in various scientific publications

Available Conjugates/Forms for CD45 Antibody (2D-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, APC-Cy7: Allophycocyanin with Cyanin-7, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD45 antibody is CD45 Antibody (35-Z6): sc-1178.