Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD42d Antibody (G-11) Alexa Fluor® 647: sc-271662 AF647. Direct fluorescent western blot analysis of CD42d expression in human platelet extract. Blocked with UltraCruz® Blocking Reagent: sc-516214. Cruz Marker™ Molecular Weight Standards detected with Cruz Marker™ MW Tag-Alexa Fluor® 488: sc-516790.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD42d CRISPR Activation Plasmid (h): sc-404793-ACT
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD42d CRISPR Activation Plasmid (h) is a synergistic activation mediator (SAM) transcription activation system designed to specifically upregulate gene expression
- CD42d CRISPR Activation Plasmid (h) consists of three plasmids at a 1:1:1 mass ratio: a plasmid encoding the deactivated Cas9 (dCas9) nuclease (D10A and N863A) fused to the transactivation domain VP64, and a blasticidin resistance gene; a plasmid encoding the MS2-p65-HSF1 fusion protein, and a hygromycin resistance gene; a plasmid encoding a target-specific 20 nt guide RNA fused to two MS2 RNA aptamers, and a puromycin resistance gene
- The resulting SAM complex binds to a site-specific region approximately 200-250 nt upstream of the transcriptional start site and provides robust recruitment of transcription factors for highly efficient gene activation
- gRNAs encoded by CD42d CRISPR Activation Plasmid (h) and CD42d CRISPR Activation Plasmid (h2) target distinct regulatory regions upstream of the GP5 transcriptional start site. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD42d Antibody (G-11): sc-271662
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD42d CRISPR Activation Plasmid (h) | sc-404793-ACT | 20 µg | $397.00 |