CD42d Antibody (G-11): sc-271662
- CD42d Antibody (G-11) is a mouse monoclonal IgG1 κ CD42d antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 17-316 mapping within an extracellular domain of CD42d of human origin
- CD42d Antibody (G-11) is recommended for detection of CD42d of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CD42d Antibody (G-11) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CD42d Antibody (G-11) for WB and IHC(P) applications. These reagents are now offered in bundles with CD42d Antibody (G-11) (see ordering information below).
QUICK LINKS
CD42d Antibody (G-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD42d protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CD42d (G-11) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD42d plays a crucial role in the early phase of primary hemostasis, where CD42d functions as part of the CD42 complex, also known as the platelet glycoprotein complex. This complex facilitates platelet adhesion to damaged blood vessel walls by binding to the von Willebrand factor, which becomes exposed on the subendothelium during vascular injury. The CD42 complex consists of four subunits: CD42b (GPIb alpha), CD42c (GPIb beta), CD42a (GPIX), and CD42d (GPV), with CD42a and CD42d being noncovalently linked to the complex. CD42d is specifically expressed in platelets and megakaryocytes, and CD42d cleavage by thrombin results in both a soluble fragment and a membrane-associated fragment, making CD42d an excellent marker for platelet activation. The gene encoding human CD42d is located on chromosome 3q29, highlighting CD42d significance in hemostatic processes and potential implications in thrombotic disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD42d Antibody (G-11) References:
- Glycoprotein V-deficient platelets have undiminished thrombin responsiveness and Do not exhibit a Bernard-Soulier phenotype. | Kahn, ML., et al. 1999. Blood. 94: 4112-21. PMID: 10590056
- GPV is a marker of in vivo platelet activation--study in a rat thrombosis model. | Ravanat, C., et al. 2000. Thromb Haemost. 83: 327-33. PMID: 10739394
- Platelet glycoprotein V binds to collagen and participates in platelet adhesion and aggregation. | Moog, S., et al. 2001. Blood. 98: 1038-46. PMID: 11493449
- Platelet GPIb-V-IX complex. Structure, function, physiology, and pathology. | Clemetson, KJ. and Clemetson, JM. 1995. Semin Thromb Hemost. 21: 130-6. PMID: 7660135
- Cloning and characterization of the gene encoding the human platelet glycoprotein V. A member of the leucine-rich glycoprotein family cleaved during thrombin-induced platelet activation. | Lanza, F., et al. 1993. J Biol Chem. 268: 20801-7. PMID: 8407908
- Human platelet glycoproteins V and IX: mapping of two leucine-rich glycoprotein genes to chromosome 3 and analysis of structures. | Yagi, M., et al. 1995. Biochemistry. 34: 16132-7. PMID: 8519770
- Genetic polymorphism in human platelet glycoprotein GP Ib/IX/V complex is enriched in GP V (CD42d). | Koskela, S., et al. 1998. Tissue Antigens. 52: 236-41. PMID: 9802603
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD42d Antibody (G-11) | sc-271662 | 200 µg/ml | $316.00 | |||
CD42d Antibody (G-11): m-IgG Fc BP-HRP Bundle | sc-527274 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD42d Antibody (G-11): m-IgG1 BP-HRP Bundle | sc-532647 | 200 µg Ab; 20 µg BP | $354.00 | |||
CD42d Antibody (G-11) AC | sc-271662 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CD42d Antibody (G-11) HRP | sc-271662 HRP | 200 µg/ml | $316.00 | |||
CD42d Antibody (G-11) FITC | sc-271662 FITC | 200 µg/ml | $330.00 | |||
CD42d Antibody (G-11) PE | sc-271662 PE | 200 µg/ml | $343.00 | |||
CD42d Antibody (G-11) Alexa Fluor® 488 | sc-271662 AF488 | 200 µg/ml | $357.00 | |||
CD42d Antibody (G-11) Alexa Fluor® 546 | sc-271662 AF546 | 200 µg/ml | $357.00 | |||
CD42d Antibody (G-11) Alexa Fluor® 594 | sc-271662 AF594 | 200 µg/ml | $357.00 | |||
CD42d Antibody (G-11) Alexa Fluor® 647 | sc-271662 AF647 | 200 µg/ml | $357.00 | |||
CD42d Antibody (G-11) Alexa Fluor® 680 | sc-271662 AF680 | 200 µg/ml | $357.00 | |||
CD42d Antibody (G-11) Alexa Fluor® 790 | sc-271662 AF790 | 200 µg/ml | $357.00 |