CD40 Double Nickase Plasmid (h): sc-400524-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD40 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD40 Double Nickase Plasmid (h) and CD40 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD40. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD40 Antibody (H-10): sc-13128
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD40 Double Nickase Plasmid (h) | sc-400524-NIC | 20 µg | $410.00 | |||
CD40 Double Nickase Plasmid (h2) | sc-400524-NIC-2 | 20 µg | $410.00 |
CD40 encodes a TNF receptor superfamily member expressed on antigen-presenting cells, including B cells, dendritic cells, and macrophages, where it integrates costimulatory cues that shape adaptive immunity. Ligation of CD40 activates canonical and non-canonical NF-κB signaling, MAPK cascades, and PI3K-dependent programs that promote cell survival, cytokine production, antigen presentation, and B-cell class-switch recombination. CD40–CD40L signaling is central to germinal center formation and T cell–dependent humoral responses, and dysregulation has been linked to immune deficiency, chronic inflammation, and B-cell malignancy biology. As a nodal immunoregulatory receptor, CD40 is frequently studied in pathways controlling APC maturation, T-cell priming, and inflammatory transcriptional networks.
CD40 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD40 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD40. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD40 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD40-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.