Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD4 CRISPR/Cas9 KO Plasmid (m): sc-419557
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD4 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD4 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD4 Antibody (MT310): sc-19641
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD4 CRISPR/Cas9 KO Plasmid (m) | sc-419557 | 20 µg | $397.00 |
Overview
Cd4 encodes CD4, a type I transmembrane glycoprotein expressed predominantly on helper T cells and a subset of thymocytes, where it serves as a co-receptor that stabilizes TCR recognition of MHC class II and promotes Lck-dependent signaling. Through its association with the TCR complex, CD4 contributes to thymic selection, T cell activation thresholds, and downstream pathways that shape differentiation into effector and regulatory lineages. Altered CD4-dependent signaling and CD4+ T cell homeostasis are central to immune dysregulation in autoimmune and inflammatory settings, and changes in CD4+ T cell function are also relevant to tumor immunology and host–pathogen interactions. In mouse models, Cd4 perturbation is widely used to dissect antigen presentation, cytokine-driven polarization, and adaptive immune network behavior in vivo and in vitro.
CD4 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd4 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd4 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd4 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD4 protein expression.
This CRISPR knockout system enables efficient generation of Cd4-deficient cell models for investigation of CD4 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd4 exon(s) critical for CD4 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd4 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD4 CRISPR/Cas9 KO Plasmid (m) and CD4 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd4 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD4 HDR Plasmid (m) and CD4 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd4 homology arms to support homology-directed repair at defined Cd4 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.