CD362/SDC2/Syndecan-2 CRISPR/Cas9 KO Plasmid (m): sc-420985

Overview

Sdc2 encodes syndecan-2 (CD362/SDC2), a transmembrane heparan sulfate proteoglycan that functions as a co-receptor for extracellular matrix components and heparin-binding growth factors. By organizing cell-surface proteoglycan–ligand interactions, SDC2 regulates cell adhesion, migration, and cytoskeletal remodeling and influences signaling through pathways such as FGF, VEGF, Wnt, and integrin/FAK–SRC networks. In mouse systems, Sdc2 has been used to study stromal–epithelial communication, angiogenic responses, and tissue remodeling in development and injury models. Altered SDC2 expression or glycosylation is also investigated in contexts of fibrosis and tumor microenvironment biology, where matrix-dependent signaling can modulate proliferation and invasiveness.

CD362/SDC2/Syndecan-2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Sdc2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Sdc2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Sdc2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD362/SDC2/Syndecan-2 protein expression.

This CRISPR knockout system enables efficient generation of Sdc2-deficient cell models for investigation of CD362/SDC2/Syndecan-2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting Sdc2 exon(s) critical for CD362/SDC2/Syndecan-2 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple Sdc2 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by CD362/SDC2/Syndecan-2 CRISPR/Cas9 KO Plasmid (m) and CD362/SDC2/Syndecan-2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Sdc2 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by CD362/SDC2/Syndecan-2 HDR Plasmid (m) and CD362/SDC2/Syndecan-2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Sdc2 homology arms to support homology-directed repair at defined Sdc2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.