Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD35 CRISPR/Cas9 KO Plasmid (h): sc-401210
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD35 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD35 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD35 Antibody (E-11): sc-7308
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD35 CRISPR/Cas9 KO Plasmid (h) | sc-401210 | 20 µg | $397.00 |
Overview
CR1 encodes CD35, a membrane complement receptor that binds C3b/C4b opsonins and promotes immune complex clearance while regulating complement activation on cell surfaces. CD35 participates in innate immune recognition and links complement to adaptive responses by modulating antigen handling and B cell signaling, and it is expressed on erythrocytes, leukocytes, and follicular dendritic cells. Through control of complement cascade amplification and phagocytic uptake, CR1 influences inflammatory homeostasis and susceptibility to complement-mediated tissue injury. Genetic variation and altered CR1/CD35 expression have been associated with immune dysregulation and inflammatory disorders, and have also been studied in contexts such as neuroinflammation and infectious disease host–pathogen interactions.
CD35 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD35 protein expression.
This CRISPR knockout system enables efficient generation of CR1-deficient cell models for investigation of CD35 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CR1 exon(s) critical for CD35 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD35 CRISPR/Cas9 KO Plasmid (h) and CD35 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD35 HDR Plasmid (h) and CD35 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CR1 homology arms to support homology-directed repair at defined CR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.