Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD33 CRISPR/Cas9 KO Plasmid (m2): sc-419543-KO-2
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD33 CRISPR/Cas9 Knockout (KO) Plasmid (m2) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD33 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD33 Antibody (6C5/2): sc-53199
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD33 CRISPR/Cas9 KO Plasmid (m2) | sc-419543-KO-2 | 20 µg | $397.00 |
Overview
Cd33 encodes CD33, a sialic acid–binding immunoglobulin-like lectin (Siglec) expressed primarily on myeloid lineage cells, where it functions as an inhibitory receptor that helps tune innate immune activation. Through immunoreceptor tyrosine-based inhibitory motifs (ITIMs), CD33 can recruit phosphatases such as SHP-1/SHP-2 to dampen signaling downstream of pattern-recognition receptors and other activating inputs, shaping cytokine production, phagocytosis, and inflammatory set points. In mouse systems, Cd33 is widely used to probe microglial and macrophage regulatory circuits, including sialylation-dependent “self” recognition and glyco-immune checkpoints. Altered CD33 activity and expression are associated with dysregulated myeloid responses in neuroinflammation and other immune-mediated disease contexts, making it a relevant node for mechanistic studies of immune homeostasis.
CD33 CRISPR/Cas9 KO Plasmid (m2) is a pool of plasmids designed for targeted disruption of the Cd33 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd33 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd33 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD33 protein expression.
This CRISPR knockout system enables efficient generation of Cd33-deficient cell models for investigation of CD33 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd33 exon(s) critical for CD33 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd33 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD33 CRISPR/Cas9 KO Plasmid (m) and CD33 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd33 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD33 HDR Plasmid (m) and CD33 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd33 homology arms to support homology-directed repair at defined Cd33 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.