Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD300A CRISPR/Cas9 KO Plasmid (h): sc-416522
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD300A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD300A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD300A Antibody (MEM-260): sc-101318
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD300A CRISPR/Cas9 KO Plasmid (h) | sc-416522 | 20 µg | $397.00 |
Overview
CD300A (also known as IRp60) encodes an immunoglobulin superfamily receptor predominantly expressed on myeloid cells, including monocytes, macrophages, and subsets of dendritic cells. Through immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic tail, CD300A recruits phosphatases such as SHP-1/2 to dampen activating signaling cascades and modulate cytokine production, phagocytosis, and immune complex responses. CD300A signaling intersects with innate immune receptor pathways, shaping thresholds for inflammatory activation and cellular crosstalk in tissue microenvironments. Altered CD300A expression or signaling has been associated with dysregulated inflammation and immune homeostasis in contexts such as autoimmune and allergic disease and tumor immunology research.
CD300A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CD300A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CD300A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CD300A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD300A protein expression.
This CRISPR knockout system enables efficient generation of CD300A-deficient cell models for investigation of CD300A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CD300A exon(s) critical for CD300A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CD300A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD300A CRISPR/Cas9 KO Plasmid (h) and CD300A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CD300A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD300A HDR Plasmid (h) and CD300A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CD300A homology arms to support homology-directed repair at defined CD300A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.