Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD3-ζ Antibody (6B10.2) Alexa Fluor® 594: sc-1239 AF594. Direct fluorescent western blot analysis of CD3-ζ expression in CCRF-CEM (A), SUP-T1 (B) and ALL-SIL (C) whole cell lysates. Blocked with UltraCruz® Blocking Reagent: sc-516214.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD3-ζ Double Nickase Plasmid (h): sc-400591-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD3-ζ Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD3-ζ Double Nickase Plasmid (h) and CD3-ζ Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD247. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD3-ζ Antibody (6B10.2): sc-1239
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD3-ζ Double Nickase Plasmid (h) | sc-400591-NIC | 20 µg | $410.00 |