CD26 Double Nickase Plasmid (h): sc-400862-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD26 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD26 Double Nickase Plasmid (h) and CD26 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DPP4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD26 Antibody (202.36): sc-7633
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD26 Double Nickase Plasmid (h) | sc-400862-NIC | 20 µg | $410.00 | |||
CD26 Double Nickase Plasmid (h2) | sc-400862-NIC-2 | 20 µg | $410.00 |
DPP4 encodes CD26, a cell-surface serine exopeptidase that removes N-terminal dipeptides from peptides with penultimate proline or alanine, thereby shaping the bioavailability and signaling range of chemokines, incretins, and other bioactive peptides. Beyond enzymatic activity, CD26 participates in immune regulation through interactions with adenosine deaminase and extracellular matrix components, influencing T cell activation, adhesion, and migration. CD26 integrates into pathways governing inflammatory trafficking, glucose homeostasis, and extracellular proteolysis, with expression and activity linked to immune-mediated disorders, metabolic dysregulation, and tumor-associated immune microenvironments. Its membrane localization and broad substrate repertoire make DPP4 a useful node for studying intercellular communication and protease-regulated signaling.
CD26 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DPP4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DPP4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DPP4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DPP4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.