CD209f CRISPR/Cas9 KO Plasmid (m): sc-427311
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD209f CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD209f genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD209f CRISPR/Cas9 KO Plasmid (m) | sc-427311 | 20 µg | $397.00 |
Overview
Cd209f encodes CD209f, a C-type lectin receptor expressed predominantly in myeloid antigen-presenting cells where it mediates recognition of glycosylated ligands and supports receptor-driven endocytosis. Through coordination of pathogen sensing, uptake, and intracellular trafficking, CD209f contributes to antigen processing and downstream regulation of innate and adaptive immune responses, including inflammatory cytokine programs. Functional links to dendritic cell and macrophage activation make Cd209f relevant to studies of host–pathogen interactions, barrier immunity, and immune tolerance mechanisms.
CD209f CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd209f gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd209f together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd209f open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD209f protein expression.
This CRISPR knockout system enables efficient generation of Cd209f-deficient cell models for investigation of CD209f signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd209f exon(s) critical for CD209f function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd209f genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD209f CRISPR/Cas9 KO Plasmid (m) and CD209f CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd209f locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD209f HDR Plasmid (m) and CD209f HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd209f homology arms to support homology-directed repair at defined Cd209f target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.