CD203c CRISPR/Cas9 KO Plasmid (m): sc-431648
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD203c CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD203c genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD203c CRISPR/Cas9 KO Plasmid (m) | sc-431648 | 20 µg | $397.00 |
Overview
Enpp3 encodes ectonucleotide pyrophosphatase/phosphodiesterase 3 (CD203c), a cell-surface ectoenzyme that hydrolyzes extracellular nucleotides to regulate purinergic signaling and the balance of pro- and anti-inflammatory mediators. In mouse immune cells, CD203c contributes to extracellular nucleotide metabolism that can influence adenosine/ATP-driven pathways shaping activation thresholds, migration, and cytokine responses. Its expression is commonly linked to basophil and mast cell biology, where it can modulate degranulation-associated programs and receptor-proximal signaling outputs. Dysregulated extracellular nucleotide processing and CD203c-associated immune activation states are relevant to models of allergy-like inflammation and other immune-mediated phenotypes.
CD203c CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Enpp3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Enpp3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Enpp3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD203c protein expression.
This CRISPR knockout system enables efficient generation of Enpp3-deficient cell models for investigation of CD203c signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Enpp3 exon(s) critical for CD203c function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Enpp3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD203c CRISPR/Cas9 KO Plasmid (m) and CD203c CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Enpp3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD203c HDR Plasmid (m) and CD203c HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Enpp3 homology arms to support homology-directed repair at defined Enpp3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.