CD203c CRISPR/Cas9 KO Plasmid (h): sc-404517
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD203c CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD203c genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD203c Antibody (1G11): sc-293363
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD203c CRISPR/Cas9 KO Plasmid (h) | sc-404517 | 20 µg | $397.00 |
Overview
ENPP3 encodes ectonucleotide pyrophosphatase/phosphodiesterase 3 (CD203c), a type II transmembrane ectoenzyme that hydrolyzes extracellular nucleotide substrates to modulate purinergic signaling and local phosphate/pyrophosphate balance. CD203c is prominently expressed on basophils and mast cells and is rapidly upregulated upon FcεRI-mediated activation, linking nucleotide metabolism with degranulation and inflammatory signaling programs. Through regulation of extracellular nucleotides, ENPP3 influences cell-surface signaling dynamics, cytokine release, and immune cell communication within tissue microenvironments. Altered ENPP3/CD203c expression has been associated with immune dysregulation and is widely used as a functional marker in studies of allergic inflammation and related immune-cell activation states.
CD203c CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the ENPP3 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the ENPP3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the ENPP3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD203c protein expression.
This CRISPR knockout system enables efficient generation of ENPP3-deficient cell models for investigation of CD203c signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting ENPP3 exon(s) critical for CD203c function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple ENPP3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD203c CRISPR/Cas9 KO Plasmid (h) and CD203c CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the ENPP3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD203c HDR Plasmid (h) and CD203c HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by ENPP3 homology arms to support homology-directed repair at defined ENPP3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.