CD2 CRISPR/Cas9 KO Plasmid (m): sc-419537
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD2 Antibody (RM2-5): sc-19639
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD2 CRISPR/Cas9 KO Plasmid (m) | sc-419537 | 20 µg | $397.00 |
Overview
Mouse Cd2 encodes CD2, an immunoglobulin superfamily adhesion and signaling receptor expressed predominantly on T cells and NK cells that mediates cell–cell contact through interaction with ligands such as CD58/CD48. CD2 participates in immunological synapse formation and co-stimulatory signaling that shapes T cell activation thresholds, cytoskeletal remodeling, and downstream pathways including Src family kinase, MAPK, and NF-κB signaling. By influencing lymphocyte adhesion, migration, and effector differentiation, CD2 contributes to the regulation of adaptive immune responses and immune homeostasis. Dysregulated CD2-dependent signaling and adhesion have been implicated in inflammatory and autoimmune-associated immune phenotypes, supporting its use in mechanistic studies of T cell function.
CD2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD2 protein expression.
This CRISPR knockout system enables efficient generation of Cd2-deficient cell models for investigation of CD2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd2 exon(s) critical for CD2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD2 CRISPR/Cas9 KO Plasmid (m) and CD2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD2 HDR Plasmid (m) and CD2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd2 homology arms to support homology-directed repair at defined Cd2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.