CD177 Antibody (C-5): sc-376329
- CD177 Antibody (C-5) is a mouse monoclonal IgG1 κ CD177 antibody, cited in 2 publications, provided at 200 µg/ml
- raised against amino acids 27-247 mapping within an internal region of CD177 of human origin
- CD177 Antibody (C-5) is recommended for detection of CD177 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CD177 Antibody (C-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CD177 Antibody (C-5) for WB and IHC(P) applications. These reagents are now offered in bundles with CD177 Antibody (C-5) (see ordering information below).
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CD177 Antibody (C-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD177 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CD177 (C-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD177, also known as NB1, is primarily located on the surface of neutrophils and plays a crucial role in the immune response by mediating the adhesion of these cells to the endothelium during inflammation. This function is vital as CD177 facilitates the migration of neutrophils to sites of infection or injury, thereby contributing to the body′s defense mechanisms. Notably, patients with polycythemia vera (PV), a neoplastic stem cell disorder characterized by excessive production of myeloid cell lines, exhibit a marked overexpression of CD177 . This upregulation is particularly significant in distinguishing PV from other erythrocytosis-related conditions, making CD177 a valuable biomarker for diagnosis and research into the underlying mechanisms of PV. Anti-CD177 antibody (C-5) effectively detects CD177 across multiple applications, enhancing research utility in both clinical and research settings, providing insights into the pathophysiology of various hematological disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CD177 Antibody (C-5) References:
- Polycythemia vera: a comprehensive review and clinical recommendations. | Tefferi, A. 2003. Mayo Clin Proc. 78: 174-94. PMID: 12583529
- Quantification of PRV-1 mRNA distinguishes polycythemia vera from secondary erythrocytosis. | Klippel, S., et al. 2003. Blood. 102: 3569-74. PMID: 12893745
- CD177 polymorphisms: correlation between high-frequency single nucleotide polymorphisms and neutrophil surface protein expression. | Caruccio, L., et al. 2004. Transfusion. 44: 77-82. PMID: 14692971
- Neutrophil CD177 (NB1 gp, HNA-2a) expression is increased in severe bacterial infections and polycythaemia vera. | Göhring, K., et al. 2004. Br J Haematol. 126: 252-4. PMID: 15238147
- Clinical significance of neutrophil CD177 mRNA expression in Ph-negative chronic myeloproliferative disorders. | Passamonti, F., et al. 2004. Br J Haematol. 126: 650-6. PMID: 15327515
- Assessment of the relative number of copies of the gene encoding human neutrophil antigen-2a(HNA-2a), CD177, and a homologous pseudogene by quantitative real-time PCR. | Dittmar, K., et al. 2003. Immunohematology. 19: 122-6. PMID: 15373677
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD177 Antibody (C-5) | sc-376329 | 200 µg/ml | $316.00 | |||
CD177 Antibody (C-5): m-IgG Fc BP-HRP Bundle | sc-529852 | 200 µg Ab; 10 µg BP | $354.00 | |||
CD177 Antibody (C-5): m-IgGκ BP-HRP Bundle | sc-522918 | 200 µg Ab, 40 µg BP | $354.00 | |||
CD177 Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-543722 | 200 µg Ab; 20 µg BP | $354.00 | |||
CD177 Antibody (C-5) AC | sc-376329 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CD177 Antibody (C-5) HRP | sc-376329 HRP | 200 µg/ml | $316.00 | |||
CD177 Antibody (C-5) FITC | sc-376329 FITC | 200 µg/ml | $330.00 | |||
CD177 Antibody (C-5) PE | sc-376329 PE | 200 µg/ml | $343.00 | |||
CD177 Antibody (C-5) Alexa Fluor® 488 | sc-376329 AF488 | 200 µg/ml | $357.00 | |||
CD177 Antibody (C-5) Alexa Fluor® 546 | sc-376329 AF546 | 200 µg/ml | $357.00 | |||
CD177 Antibody (C-5) Alexa Fluor® 594 | sc-376329 AF594 | 200 µg/ml | $357.00 | |||
CD177 Antibody (C-5) Alexa Fluor® 647 | sc-376329 AF647 | 200 µg/ml | $357.00 | |||
CD177 Antibody (C-5) Alexa Fluor® 680 | sc-376329 AF680 | 200 µg/ml | $357.00 | |||
CD177 Antibody (C-5) Alexa Fluor® 790 | sc-376329 AF790 | 200 µg/ml | $357.00 |