CD163 CRISPR/Cas9 KO Plasmid (m): sc-429989
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD163 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD163 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD163 Antibody (ED2): sc-58965
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD163 CRISPR/Cas9 KO Plasmid (m) | sc-429989 | 20 µg | $397.00 |
Overview
Cd163 encodes CD163, a macrophage- and monocyte-enriched scavenger receptor that binds hemoglobin–haptoglobin complexes and supports their endocytosis and clearance, linking iron recycling to resolution-phase inflammatory programs. CD163 participates in innate immune regulation through endocytic trafficking and modulation of cytokine responses, and is commonly associated with alternatively activated macrophage phenotypes in tissue repair contexts. In mouse models, CD163-positive myeloid populations are used to study inflammatory microenvironments, macrophage polarization, and myeloid–stromal crosstalk in chronic inflammatory and fibrotic conditions. Altered Cd163 expression has also been leveraged as a marker of disease-relevant macrophage states in settings such as infection, metabolic inflammation, and tumor-associated myeloid biology.
CD163 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd163 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd163 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd163 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD163 protein expression.
This CRISPR knockout system enables efficient generation of Cd163-deficient cell models for investigation of CD163 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd163 exon(s) critical for CD163 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd163 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD163 CRISPR/Cas9 KO Plasmid (m) and CD163 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd163 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD163 HDR Plasmid (m) and CD163 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd163 homology arms to support homology-directed repair at defined Cd163 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.