CD163 Antibody (RM3/1): sc-33715

CD163 Antibody (RM3/1) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect human CD163 with exceptional precision through immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). CD163, also known as M130 or SCARI1, is a pivotal macrophage-associated antigen within the scavenger receptor cysteine-rich (SRCR) superfamily, predominantly localized on the surface of macrophages and, to a lesser extent, on monocytes. This cell surface localization enables CD163 to function effectively as a receptor that mediates the scavenging and endocytosis of haptoglobin-hemoglobin complexes, thereby playing a significant role in anti-inflammatory response and iron metabolism. The membrane-bound position of CD163 facilitates interaction with extracellular haptoglobin-hemoglobin, preventing oxidative stress and maintaining cellular homeostasis during hemolytic events. Anti-CD163 antibody (RM3/1) is available in both non-conjugated form and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatile options for diverse experimental applications. The receptor-ligand binding of CD163 is calcium-dependent and exhibits high affinity, and multiple CD163 isoforms, which vary in cytoplasmic domains and potential phosphorylation sites, suggest distinct signaling mechanisms that regulate macrophage function. The gene encoding CD163 is located on human chromosome 12p13.31, highlighting genetic significance and potential implications in various inflammatory and immune-mediated diseases. This comprehensive characterization of CD163 underscores importance in cellular processes and validates CD163 (RM3/1) antibody as an essential tool for researchers studying macrophage biology and related pathways.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD163 Antibody (RM3/1) has 16 citations in a variety of scientific publications.

Primary antibodies like Anti-CD163 Antibody (RM3/1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD163 Antibody (RM3/1) Specifications

Alternate Product Name: CD163

Product Clone Name: RM3/1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD163

Epitope Species: human

Product Reactivity/Detection: CD163

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 16 citations in various scientific publications

Available Conjugates/Forms for CD163 Antibody (RM3/1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD163 antibody is CD163 Antibody (GHI/61): sc-20066.