CD163 Antibody (GHI/61): sc-20066

CD163 Antibody (GHI/61) is a mouse monoclonal IgG1 kappa light chain antibody targeting human CD163 protein, making anti-CD163 antibody (GHI/61) an essential tool for researchers studying macrophage biology and related pathways. CD163, also known as M130 or SCARI1, is a member of the scavenger receptor cysteine-rich (SRCR) superfamily and serves as a critical marker for macrophages and, to a lesser extent, monocytes. CD163 plays a vital role in the scavenging of hemoglobin-haptoglobin complexes, mediating their endocytosis through a calcium-dependent, high-affinity interaction that ensures efficient clearance of free hemoglobin, thereby preventing oxidative stress and inflammation. CD163 receptor is not only involved in hemoglobin clearance but also participates in anti-inflammatory responses, making CD163 an important player in immune regulation and a potential therapeutic target for various inflammatory diseases. CD163 monoclonal antibody (GHI/61) is recommended for use in multiple applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM), ensuring versatility in diverse experimental setups. Available in both non-conjugated form and a range of conjugated versions such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® dyes, anti-CD163 antibody (GHI/61) facilitates precise detection and visualization of CD163 in different assay conditions. Multiple CD163 isoforms, which vary in cytoplasmic domains and phosphorylation sites, suggest diverse signaling mechanisms, further underscoring the importance of accurately detecting and studying CD163. Encoded by the gene located on human chromosome 12p13.31, CD163′s expression and regulation are pivotal for maintaining immune homeostasis and understanding CD163′s role can provide deeper insights into macrophage-mediated processes in health and disease.

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CD163 Antibody (GHI/61) References:

1. Genomic organization and chromosomal localization of the human CD163 (M130) gene: a member of the scavenger receptor cysteine-rich superfamily.  |  Ritter, M., et al. 1999. Biochem Biophys Res Commun. 260: 466-74. PMID: 10403791
2. Regulation of CD 163 on human macrophages: cross-linking of CD163 induces signaling and activation.  |  Van den Heuvel, MM., et al. 1999. J Leukoc Biol. 66: 858-66. PMID: 10577520
3. Regulation of scavenger receptor CD163 expression in human monocytes and macrophages by pro- and antiinflammatory stimuli.  |  Buechler, C., et al. 2000. J Leukoc Biol. 67: 97-103. PMID: 10648003
4. Human monocytes express CD163, which is upregulated by IL-10 and identical to p155.  |  Sulahian, TH., et al. 2000. Cytokine. 12: 1312-21. PMID: 10975989
5. Assignment of CD163B, the gene encoding M160, a novel scavenger receptor, to human chromosome 12p13.3 by in situ hybridization and somatic cell hybrid analysis.  |  Stover, CM., et al. 2000. Cytogenet Cell Genet. 90: 246-7. PMID: 11124526
6. Identification of the haemoglobin scavenger receptor.  |  Kristiansen, M., et al. 2001. Nature. 409: 198-201. PMID: 11196644
7. Interaction of CD163 with the regulatory subunit of casein kinase II (CKII) and dependence of CD163 signaling on CKII and protein kinase C.  |  Ritter, M., et al. 2001. Eur J Immunol. 31: 999-1009. PMID: 11298324
8. A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily.  |  Law, SK., et al. 1993. Eur J Immunol. 23: 2320-5. PMID: 8370408

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD163 Antibody (GHI/61) has 48 citations in a variety of scientific publications.

Primary antibodies like Anti-CD163 Antibody (GHI/61) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD163 Antibody (GHI/61) Specifications

Alternate Product Name: CD163

Product Clone Name: GHI/61

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD163

Epitope Species: human

Product Reactivity/Detection: CD163

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 48 citations in various scientific publications

Available Conjugates/Forms for CD163 Antibody (GHI/61): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin