CD16 Antibody (3G8): sc-19620

CD16 Antibody (3G8) is a mouse monoclonal IgG1 kappa light chain antibody, engineered to detect human CD16, also known as Fc gamma receptor III (FcγRIII), through immunofluorescence (IF) and flow cytometry (FCM) applications. CD16 exists in two main isoforms: CD16A (FcγRIIIA), a transmembrane receptor expressed on natural killer (NK) cells and macrophages, and CD16B (FcγRIIIB), a glycosylphosphatidylinositol (GPI)-anchored protein found on neutrophils. CD16 (3G8) antibody binds the Fc region of IgG antibodies, thereby mediating antibody-dependent cellular cytotoxicity (ADCC) and promoting the phagocytosis of antibody-coated pathogens or cells. The functionality of CD16A relies on its association with the γ subunit of the FcεRI or the ζ subunit of the TCR-CD3 complex, which is crucial for expression on the cell surface. CD16 is unique among eukaryotic receptors because CD16 exists both as a transmembrane and a GPI-anchored isoform, highlighting versatile roles in immune responses. Anti-CD16 antibody (3G8) is available in various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® variants, as well as in a non-conjugated format, making CD16 (3G8) monoclonal antibody adaptable for a wide range of experimental applications. CD16 monoclonal antibody (3G8) is vital for research into immune system dynamics, autoimmune disorders, and diseases such as paroxysmal nocturnal hemoglobinuria (PNH), where alterations in CD16 expression can have significant pathological implications. With alternate names including FcγRIII, Fc gamma receptor III, CD16A, and CD16B, CD16 (3G8) antibody provides comprehensive detection capabilities for studying the distinct isoforms and their respective functions within the immune milieu.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD16 Antibody (3G8) has 25 citations in a variety of scientific publications.

Primary antibodies like Anti-CD16 Antibody (3G8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD16 Antibody (3G8) Specifications

Alternate Product Name: CD16

Product Clone Name: 3G8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids:

Epitope Target: CD16

Epitope Species: human

Product Reactivity/Detection: CD16

Species Reactivity/Detection: human

Additional Mammalian Reactivity: primates

Additional Non-Mammalian Reactivity: N/A

Applications: IF, FCM

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 25 citations in various scientific publications

Available Conjugates/Forms for CD16 Antibody (3G8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD16 antibody is CD16 Antibody (DJ130c): sc-20052.