CD15s Antibody (CHO131): sc-32243

CD15s Antibody (CHO131) is a mouse monoclonal IgM antibody that detects CD15s protein of human origin by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Anti-CD15s antibody (CHO131) is available in both non-conjugated and various conjugated forms, including phycoerythrin (PE) and fluorescein isothiocyanate (FITC). CD15s protein plays a crucial role in cellular interactions, particularly in the context of selectins, which are a family of carbohydrate-binding proteins essential for mediating the adhesion of leukocytes to the endothelium during inflammation and immune responses. This interaction is vital for the proper trafficking of leukocytes from the bloodstream to lymphoid organs and sites of inflammation, thereby influencing immune surveillance and response. Notably, CD15s is associated with sialylated Lewis glycan structures, such as sialyl-Lewis X, which are often overexpressed in various carcinomas, including colorectal adenocarcinomas and metastatic liver tumors. The presence of CD15s, particularly in the form of core 2 O-glycans, is linked to tumor progression and metastasis, making CD15s a significant tumor-associated antigen and a potential early predictor of metastatic disease. CD15s monoclonal antibody (CHO131) enables valuable insights into the mechanisms of leukocyte trafficking and the role of selectins in cancer biology.

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CD15s Antibody (CHO131) References:

1. The monoclonal antibody CHO-131 binds to a core 2 O-glycan terminated with sialyl-Lewis x, which is a functional glycan ligand for P-selectin.  |  Walcheck, B., et al. 2002. Blood. 99: 4063-9. PMID: 12010808
2. Extended core 1 and core 2 branched O-glycans differentially modulate sialyl Lewis X-type L-selectin ligand activity.  |  Mitoma, J., et al. 2003. J Biol Chem. 278: 9953-61. PMID: 12529363
3. The selectin family of carbohydrate-binding proteins: structure and importance of carbohydrate ligands for cell adhesion.  |  Cummings, RD. and Smith, DF. 1992. Bioessays. 14: 849-56. PMID: 1285423
4. Expression of the high-affinity selectin glycan ligand C2-O-sLeX by colon carcinoma cells.  |  St Hill, CA., et al. 2005. Cancer Lett. 217: 105-13. PMID: 15596301
5. The high affinity selectin glycan ligand C2-O-sLex and mRNA transcripts of the core 2 beta-1,6-N-acetylglucosaminyltransferase (C2GnT1) gene are highly expressed in human colorectal adenocarcinomas.  |  St Hill, CA., et al. 2009. BMC Cancer. 9: 79. PMID: 19267921
6. C2-O-sLeX glycoproteins are E-selectin ligands that regulate invasion of human colon and hepatic carcinoma cells.  |  St Hill, CA., et al. 2011. PLoS One. 6: e16281. PMID: 21283832
7. CD15s is a potential biomarker of serious bacterial infection in infants admitted to hospital.  |  Markic, J., et al. 2013. Eur J Pediatr. 172: 1363-9. PMID: 23728509
8. Thieno[2,3-b]Pyridine Derivative Targets Epithelial, Mesenchymal and Hybrid CD15s+ Breast Cancer Cells.  |  Marijan, S., et al. 2021. Medicines (Basel). 8: PMID: 34206154
9. Prognostic and Therapeutic Role of CD15 and CD15s in Cancer.  |  Szlasa, W., et al. 2022. Cancers (Basel). 14: PMID: 35565333
10. Multivalent sialyl-LeX: potent inhibitors of E-selectin-mediated cell adhesion; reagent for staining activated endothelial cells.  |  Welply, JK., et al. 1994. Glycobiology. 4: 259-65. PMID: 7524825
11. Higher-affinity oligosaccharide ligands for E-selectin.  |  Nelson, RM., et al. 1993. J Clin Invest. 91: 1157-66. PMID: 7680663
12. Studies on selectin-carbohydrate interactions.  |  Jacob, GS., et al. 1995. Adv Exp Med Biol. 376: 283-90. PMID: 8597260

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD15s Antibody (CHO131) has 6 citations in a variety of scientific publications.

Primary antibodies like Anti-CD15s Antibody (CHO131) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD15s Antibody (CHO131) Specifications

Alternate Product Name: CD15s

Product Clone Name: CHO131

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: CD15s

Epitope Species: human

Product Reactivity/Detection: CD15s

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), FCM

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 6 citations in various scientific publications

Available Conjugates/Forms for CD15s Antibody (CHO131): FITC: Fluoroscein, PE: Phycoerythrin