CD15s Antibody (5F18): sc-70545

CD15s Antibody (5F18) is a mouse monoclonal IgM antibody that detects CD15s in human samples through flow cytometry (FCM). CD15s, also known as sialyl-Lewis X, is a carbohydrate determinant playing a crucial role in mediating cellular interactions, particularly in leukocyte trafficking to lymphoid organs and sites of inflammation. CD15s is predominantly expressed on the surface of certain cancer cells, including colorectal adenocarcinomas and metastatic liver tumors, where CD15s is associated with the presence of core 2 O-glycans. These glycan structures, synthesized by the enzyme core 2 beta1,6 N-acetylglucosaminyltransferase (C2GnT1), serve as high-affinity ligands for selectins, facilitating the adhesion and migration of leukocytes. The upregulation of CD15s in tumor cells suggests CD15s potential as a tumor-associated antigen, making CD15s a valuable biomarker for early detection of metastasis. Using anti-CD15s antibody (5F18) in research can provide insights into cancer progression mechanisms and selectin roles in immune response, enhancing understanding of both normal physiology and pathological conditions.

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CD15s Antibody (5F18) References:

1. A cloned CD15s-negative variant of HL60 cells is deficient in expression of FUT7 and does not adhere to cytokine-stimulated endothelial cells.  |  Weston, BW., et al. 1999. Eur J Haematol. 63: 42-9. PMID: 10414454
2. Clinical utility of monitoring sialyl Lewis(X) (CD15S) antigen on peripheral lymphocytes for the diagnosis and treatment of rejection after renal transplantation.  |  Ishida, H., et al. 2000. Transplantation. 69: 59-63. PMID: 10653381
3. Sialyl Lewis(x) (CD15s) monitoring as a means to select antirejection therapy in patients with rejection after renal transplantation: CD15s monitoring for treatment and diagnosis in patients with acute rejection after renal transplantation.  |  Ishida, H., et al. 2000. Transplantation. 69: 2208-11. PMID: 10852628
4. Prognostic role of serum sialyl Lewisx (CD15s) in colorectal cancer.  |  Paganuzzi, M., et al. 2003. Oncology. 65: 52-9. PMID: 12837983
5. Differential expression of the carcinoembryonic antigen-related cell adhesion molecules panCD66, CD66a, CD66c and of sialyl-Lewis x (CD15s) on blast cells of acute leukemias.  |  Ratei, R., et al. 2008. Int J Hematol. 87: 137-143. PMID: 18299959
6. Sialyl Lewis x (CD15s) identifies highly differentiated and most suppressive FOXP3high regulatory T cells in humans.  |  Miyara, M., et al. 2015. Proc Natl Acad Sci U S A. 112: 7225-30. PMID: 26015572
7. Hyperbaric environment up-regulates CD15s expression on leukocytes, down-regulates CD77 expression on renal cells and up-regulates CD34 expression on pulmonary and cardiac cells in rat.  |  Đevenica, D., et al. 2016. Iran J Basic Med Sci. 19: 821-828. PMID: 27746862
8. CD15s/CD62E Interaction Mediates the Adhesion of Non-Small Cell Lung Cancer Cells on Brain Endothelial Cells: Implications for Cerebral Metastasis.  |  Jassam, SA., et al. 2017. Int J Mol Sci. 18: PMID: 28698503
9. Fucosyltransferase 4 and 7 mediates adhesion of non-small cell lung cancer cells to brain-derived endothelial cells and results in modification of the blood-brain-barrier: in vitro investigation of CD15 and CD15s in lung-to-brain metastasis.  |  Jassam, SA., et al. 2019. J Neurooncol. 143: 405-415. PMID: 31104223
10. A major role for CD62P/CD15s interaction in leukocyte margination during hemodialysis.  |  Stuard, S., et al. 1995. Kidney Int. 48: 93-102. PMID: 7564097

The preferred CD15s antibody is CD15s Antibody (CHO131): sc-32243.