CD155 Double Nickase Plasmid (h): sc-404597-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD155 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD155 Double Nickase Plasmid (h) and CD155 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PVR. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD155 Antibody (SKII.4): sc-53572
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD155 Double Nickase Plasmid (h) | sc-404597-NIC | 20 µg | $410.00 | |||
CD155 Double Nickase Plasmid (h2) | sc-404597-NIC-2 | 20 µg | $410.00 |
PVR encodes CD155, an immunoglobulin superfamily cell-surface adhesion molecule that functions as a ligand for DNAM-1 (CD226), TIGIT, and CD96 to modulate cytotoxic lymphocyte activation and immune checkpoint signaling. CD155 also participates in cell–cell and cell–matrix interactions, influencing actin remodeling, migration, and contact inhibition through pathways linked to focal adhesion and cytoskeletal dynamics. Altered PVR/CD155 expression has been associated with tumor immune evasion, invasion, and metastatic behavior, and it is frequently examined in the context of immune surveillance within the tumor microenvironment. In addition, CD155 serves as a receptor for poliovirus, making it relevant to studies of viral binding, entry, and host–pathogen interactions in human cells.
CD155 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PVR locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PVR. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PVR function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PVR-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.