Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CD151 CRISPR/Cas9 KO Plasmid (h): sc-401969
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD151 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD151 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD151 Antibody (H-8): sc-271216
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD151 CRISPR/Cas9 KO Plasmid (h) | sc-401969 | 20 µg | $397.00 |
Overview
CD151 (tetraspanin-24) is a four-transmembrane tetraspanin that organizes tetraspanin-enriched microdomains and scaffolds adhesion and signaling complexes at the plasma membrane. By partnering with laminin-binding integrins such as ITGA3/ITGB1 and ITGA6/ITGB4, CD151 regulates cell–matrix adhesion, spreading, and motility, and modulates downstream signaling through focal adhesion and cytoskeletal remodeling pathways. CD151 also influences membrane trafficking and exosome biology, shaping receptor distribution and signal propagation. Dysregulated CD151 expression or function has been associated with altered epithelial integrity and invasive phenotypes in cancer biology, as well as kidney and vascular pathophysiology, making it a useful target for mechanistic studies.
CD151 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CD151 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CD151 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CD151 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD151 protein expression.
This CRISPR knockout system enables efficient generation of CD151-deficient cell models for investigation of CD151 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CD151 exon(s) critical for CD151 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CD151 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD151 CRISPR/Cas9 KO Plasmid (h) and CD151 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CD151 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD151 HDR Plasmid (h) and CD151 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CD151 homology arms to support homology-directed repair at defined CD151 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.