CD133 Antibody (E-11): sc-365537

CD133 Antibody (E-11) is a mouse monoclonal IgG1 kappa light chain antibody that detects CD133 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CD133 antibody (E-11) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD133, also known as PROM1 or Prominin-1, is a pentaspan transmembrane glycoprotein that plays a crucial role in maintaining and regulating stem cell populations. CD133 is predominantly expressed on primitive hematopoietic stem cells, progenitor cells, retinoblastoma cells, hemangioblasts, and neural stem cells, as well as in developing epithelial tissues. CD133′s unique structure, which includes an extracellular N-terminus, two short intracellular loops, two large extracellular loops, and an intracellular C-terminus, is essential for cellular signaling and membrane organization. CD133 is implicated in forming plasma membrane protrusions, which are critical for proper retinal cell functioning, as these protrusions facilitate the shedding of photoreceptive membranes that are subsequently phagocytosed by the retinal pigment epithelium. This process is vital for maintaining retinal health and may be linked to inherited retinal degenerations, making CD133 a significant marker for research in stem cell biology and retinal diseases. CD133 gene is located on chromosome 4p15.32 and encodes an 865 amino acid protein, underscoring its importance in both developmental biology and potential therapeutic applications.

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CD133 Antibody (E-11) References:

1. CD133+ cell selection is an alternative to CD34+ cell selection for ex vivo expansion of hematopoietic stem cells.  |  Kobari, L., et al. 2001. J Hematother Stem Cell Res. 10: 273-81. PMID: 11359674
2. Human trophoblast contains an intracellular protein reactive with an antibody against CD133--a novel marker for trophoblast.  |  Pötgens, AJ., et al. 2001. Placenta. 22: 639-45. PMID: 11504532
3. CD133(+) cells isolated from various sources and their role in future clinical perspectives.  |  Meregalli, M., et al. 2010. Expert Opin Biol Ther. 10: 1521-8. PMID: 20932225
4. CD133+ cells as a therapeutic target for kidney diseases.  |  Bussolati, B., et al. 2012. Expert Opin Ther Targets. 16: 157-65. PMID: 22324879
5. CD133: beyond a cancer stem cell biomarker.  |  Barzegar Behrooz, A., et al. 2019. J Drug Target. 27: 257-269. PMID: 29911902
6. New emerging roles of CD133 in cancer stem cell: Signaling pathway and miRNA regulation.  |  Aghajani, M., et al. 2019. J Cell Physiol. 234: 21642-21661. PMID: 31102292
7. The role of CD133 in hepatocellular carcinoma.  |  Liu, F. and Qian, Y. 2021. Cancer Biol Ther. 22: 291-300. PMID: 33899676
8. CD133(+)HIF-1α(-) Expression After Chemoradiotherapy Predicts Poor Prognosis in Rectal Cancer.  |  Tachikawa, Y., et al. 2022. Anticancer Res. 42: 2033-2043. PMID: 35347026
9. Can CD133 Be Regarded as a Prognostic Biomarker in Oncology: Pros and Cons.  |  Gisina, A., et al. 2023. Int J Mol Sci. 24: PMID: 38139228
10. Interconnection of CD133 Stem Cell Marker with Autophagy and Apoptosis in Colorectal Cancer.  |  Sipos, F. and Műzes, G. 2024. Int J Mol Sci. 25: PMID: 39456981
11. AC133, a novel marker for human hematopoietic stem and progenitor cells.  |  Yin, AH., et al. 1997. Blood. 90: 5002-12. PMID: 9389720
12. A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning.  |  Miraglia, S., et al. 1997. Blood. 90: 5013-21. PMID: 9389721

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD133 Antibody (E-11) has 12 citations in a variety of scientific publications.

Primary antibodies like Anti-CD133 Antibody (E-11) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD133 Antibody (E-11) Specifications

Alternate Product Name: PROM1

Product Clone Name: E-11

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 841-865

Epitope Target: CD133

Epitope Species: human

Product Reactivity/Detection: CD133

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 12 citations in various scientific publications

Available Conjugates/Forms for CD133 Antibody (E-11): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin