CD109 CRISPR/Cas9 KO Plasmid (m): sc-433467
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD109 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD109 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD109 Antibody (C-9): sc-271085
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD109 CRISPR/Cas9 KO Plasmid (m) | sc-433467 | 20 µg | $397.00 |
Overview
Cd109 encodes CD109, a glycosylphosphatidylinositol-anchored cell-surface glycoprotein that modulates transforming growth factor-β (TGF-β) signaling by influencing receptor availability and downstream SMAD-dependent transcription. In mouse cells, CD109 has been linked to regulation of epithelial and stromal cell behavior, including control of proliferation, differentiation, and extracellular matrix remodeling. By tuning TGF-β pathway output, CD109 can impact processes such as immune regulation, wound responses, and fibrosis-associated signaling networks. Altered CD109 expression and signaling balance are frequently examined in contexts of dysregulated growth control and tumor-associated microenvironment phenotypes in preclinical models.
CD109 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cd109 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cd109 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cd109 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD109 protein expression.
This CRISPR knockout system enables efficient generation of Cd109-deficient cell models for investigation of CD109 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cd109 exon(s) critical for CD109 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cd109 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD109 CRISPR/Cas9 KO Plasmid (m) and CD109 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cd109 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD109 HDR Plasmid (m) and CD109 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cd109 homology arms to support homology-directed repair at defined Cd109 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.