Ccr1l1 CRISPR/Cas9 KO Plasmid (m): sc-419703
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Ccr1l1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Ccr1l1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ccr1l1 CRISPR/Cas9 KO Plasmid (m) | sc-419703 | 20 µg | $397.00 |
Overview
Ccr1l1 encodes a CCR1-like chemokine receptor–related protein predicted to participate in leukocyte trafficking and inflammatory signaling networks that are typically governed by G protein–coupled receptor (GPCR) chemokine axes. Although less well characterized than canonical CCR family members, Ccr1l1 is positioned to influence chemokine-directed migration, immune cell localization, and downstream pathways linked to actin remodeling and cytokine/chemokine circuit regulation. These processes are central to innate and adaptive immune responses and are frequently implicated in models of inflammatory disease, infection, and tumor–immune microenvironment dynamics. Mouse Ccr1l1 therefore provides a useful entry point for probing chemokine-receptor–adjacent biology and immunoregulatory mechanisms in vivo and in primary cell systems.
Ccr1l1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ccr1l1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ccr1l1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ccr1l1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Ccr1l1 protein expression.
This CRISPR knockout system enables efficient generation of Ccr1l1-deficient cell models for investigation of Ccr1l1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ccr1l1 exon(s) critical for Ccr1l1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ccr1l1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Ccr1l1 CRISPR/Cas9 KO Plasmid (m) and Ccr1l1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ccr1l1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Ccr1l1 HDR Plasmid (m) and Ccr1l1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ccr1l1 homology arms to support homology-directed repair at defined Ccr1l1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.