Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CCL14 CRISPR/Cas9 KO Plasmid (h): sc-406423
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCL14 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CCL14 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CCL14 Antibody (E-12): sc-376152
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCL14 CRISPR/Cas9 KO Plasmid (h) | sc-406423 | 20 µg | $397.00 |
Overview
CCL14 encodes a secreted CC chemokine that helps coordinate leukocyte trafficking by engaging chemokine receptors on monocytes, lymphocytes, and other immune cell subsets, thereby shaping inflammatory gradients and immune cell positioning within tissues. As part of the broader chemokine network, CCL14 contributes to chemotaxis, modulation of cytokine signaling, and cross-talk with pathways governing endothelial activation and extracellular matrix remodeling. Dysregulated chemokine signaling involving CCL14 has been linked to altered immune infiltration patterns observed in chronic inflammatory states and tumor-associated inflammation, where shifts in chemokine cues can influence immune surveillance and stromal interactions. Accordingly, CCL14 is frequently studied in contexts such as immune cell migration assays, inflammatory signaling profiling, and systems-level mapping of chemokine networks.
CCL14 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CCL14 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CCL14 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CCL14 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CCL14 protein expression.
This CRISPR knockout system enables efficient generation of CCL14-deficient cell models for investigation of CCL14 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CCL14 exon(s) critical for CCL14 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CCL14 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CCL14 CRISPR/Cas9 KO Plasmid (h) and CCL14 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CCL14 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CCL14 HDR Plasmid (h) and CCL14 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CCL14 homology arms to support homology-directed repair at defined CCL14 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.