CCDC90A CRISPR/Cas9 KO Plasmid (h): sc-406721
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCDC90A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CCDC90A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCDC90A CRISPR/Cas9 KO Plasmid (h) | sc-406721 | 20 µg | $397.00 |
Overview
MCUR1 (also known as CCDC90A) encodes a mitochondrial inner membrane protein that supports mitochondrial calcium uptake by modulating the mitochondrial calcium uniporter complex. Through regulation of matrix Ca²⁺ levels, CCDC90A links calcium signaling to oxidative phosphorylation, TCA cycle enzyme activity, and cellular bioenergetic homeostasis, with downstream effects on reactive oxygen species and stress responses. Perturbation of MCUR1/CCDC90A function has been associated with altered mitochondrial metabolism and vulnerability to mitochondrial dysfunction, making it relevant to studies of cardiometabolic stress, neurobiology, and cancer cell metabolic adaptation. As a mitochondrial signaling node, it is frequently interrogated in pathways connecting calcium handling, apoptosis susceptibility, and mitochondrial quality control.
CCDC90A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the MCUR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the MCUR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the MCUR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CCDC90A protein expression.
This CRISPR knockout system enables efficient generation of MCUR1-deficient cell models for investigation of CCDC90A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting MCUR1 exon(s) critical for CCDC90A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple MCUR1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CCDC90A CRISPR/Cas9 KO Plasmid (h) and CCDC90A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the MCUR1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CCDC90A HDR Plasmid (h) and CCDC90A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by MCUR1 homology arms to support homology-directed repair at defined MCUR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.