CCDC57 CRISPR/Cas9 KO Plasmid (m): sc-427913
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCDC57 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CCDC57 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCDC57 CRISPR/Cas9 KO Plasmid (m) | sc-427913 | 20 µg | $397.00 |
Overview
Ccdc57 encodes the coiled-coil domain–containing protein CCDC57, a predicted cytoskeletal and organelle-associated factor implicated in organizing microtubule-dependent processes. Coiled-coil proteins frequently act as scaffolds that support centrosome integrity, intracellular trafficking, and cell-cycle progression, linking CCDC57 to pathways governing mitotic spindle dynamics and spatial regulation of signaling. In mouse systems, perturbation of proteins in these networks is commonly studied for effects on proliferation, genome stability, and cellular differentiation. As such, CCDC57 is relevant to mechanistic research areas that intersect with developmental phenotypes and disease-associated defects in cytoskeletal organization and mitotic control.
CCDC57 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Ccdc57 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Ccdc57 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Ccdc57 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CCDC57 protein expression.
This CRISPR knockout system enables efficient generation of Ccdc57-deficient cell models for investigation of CCDC57 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Ccdc57 exon(s) critical for CCDC57 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Ccdc57 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CCDC57 CRISPR/Cas9 KO Plasmid (m) and CCDC57 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Ccdc57 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CCDC57 HDR Plasmid (m) and CCDC57 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Ccdc57 homology arms to support homology-directed repair at defined Ccdc57 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.