CCDC111 CRISPR/Cas9 KO Plasmid (h): sc-404310
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CCDC111 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CCDC111 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CCDC111 CRISPR/Cas9 KO Plasmid (h) | sc-404310 | 20 µg | $397.00 |
Overview
PRIMPOL (also known as CCDC111) encodes PrimPol, a primase–polymerase that promotes DNA damage tolerance by repriming DNA synthesis downstream of lesions and assisting replication fork restart. This activity supports genome stability during S phase and intersects with pathways involved in replication stress responses, post-replication repair, and maintenance of mitochondrial and nuclear DNA integrity. Dysregulated replication stress and impaired lesion bypass are linked to mutational accumulation and chromosomal instability, making PRIMPOL a relevant target for studying mechanisms that influence cancer-associated genome maintenance. In addition, PRIMPOL function is often examined in the context of oxidative damage, UV-induced lesions, and polymerase switching events during stalled fork recovery.
CCDC111 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PRIMPOL gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PRIMPOL together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PRIMPOL open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CCDC111 protein expression.
This CRISPR knockout system enables efficient generation of PRIMPOL-deficient cell models for investigation of CCDC111 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PRIMPOL exon(s) critical for CCDC111 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PRIMPOL genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CCDC111 CRISPR/Cas9 KO Plasmid (h) and CCDC111 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PRIMPOL locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CCDC111 HDR Plasmid (h) and CCDC111 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PRIMPOL homology arms to support homology-directed repair at defined PRIMPOL target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.