CBX7 Double Nickase Plasmid (h): sc-402903-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CBX7 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CBX7 Double Nickase Plasmid (h) and CBX7 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CBX7. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CBX7 Antibody (G-3): sc-376274
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CBX7 Double Nickase Plasmid (h) | sc-402903-NIC | 20 µg | $410.00 | |||
CBX7 Double Nickase Plasmid (h2) | sc-402903-NIC-2 | 20 µg | $410.00 |
CBX7 (chromobox 7) encodes a Polycomb group chromodomain protein that recognizes H3K27me3 and helps assemble canonical PRC1 complexes to promote chromatin compaction and stable transcriptional repression. Through regulation of developmental gene programs, cell cycle checkpoints, and cellular senescence, CBX7 contributes to maintenance of epigenetic memory and lineage-specific transcriptional states. Dysregulated CBX7 activity has been associated with altered differentiation and proliferative control in multiple disease contexts, including cancers, where shifts in Polycomb-mediated silencing can remodel oncogenic and tumor suppressor networks. As a chromatin reader within Polycomb pathways, CBX7 is frequently studied to dissect how histone marks are interpreted to control genome-wide transcriptional outputs.
CBX7 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CBX7 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CBX7. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CBX7 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CBX7-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.