CB1/Cannabinoid Receptor 1/CNR1 CRISPR/Cas9 KO Plasmid (h2): sc-400648-KO-2

Overview

CNR1 encodes cannabinoid receptor 1 (CB1), a predominantly neuronal G protein–coupled receptor that transduces endocannabinoid signals to regulate neurotransmitter release and synaptic plasticity. CB1 primarily couples to Gi/o proteins to inhibit adenylyl cyclase, modulate cAMP/PKA signaling, and engage MAPK/ERK pathways while influencing ion channel activity and presynaptic calcium dynamics. Through these mechanisms, CB1 contributes to circuit-level control of reward, nociception, appetite, stress responsivity, and neuroinflammation. Dysregulated CNR1 signaling has been associated with neuropsychiatric phenotypes and metabolic traits, supporting its use as a molecular node for studying brain–body signaling and homeostatic control.

CB1/Cannabinoid Receptor 1/CNR1 CRISPR/Cas9 KO Plasmid (h2) is a pool of plasmids designed for targeted disruption of the CNR1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CNR1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.

The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CNR1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CB1/Cannabinoid Receptor 1/CNR1 protein expression.

This CRISPR knockout system enables efficient generation of CNR1-deficient cell models for investigation of CB1/Cannabinoid Receptor 1/CNR1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.

Key Features

• sgRNAs targeting CNR1 exon(s) critical for CB1/Cannabinoid Receptor 1/CNR1 function
• Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
• GFP reporter for identification of transfected cells
• Pool of plasmids targeting multiple CNR1 genomic sites to improve knockout efficiency
• Compatible with delivery by transfection

Design Variants

CRISPRs +/- HDRs

• gRNAs encoded by CB1/Cannabinoid Receptor 1/CNR1 CRISPR/Cas9 KO Plasmid (h) and CB1/Cannabinoid Receptor 1/CNR1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CNR1 locus. One or both targeting designs may be available. See Related Products for availability.
• HDR donor constructs encoded by CB1/Cannabinoid Receptor 1/CNR1 HDR Plasmid (h) and CB1/Cannabinoid Receptor 1/CNR1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CNR1 homology arms to support homology-directed repair at defined CNR1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.