cathepsin H Antibody (F-7): sc-398527

Cathepsin H Antibody (F-7) is a mouse monoclonal IgG1 kappa light chain antibody that detects cathepsin H of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-cathepsin H antibody (F-7) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cathepsin H, also known as N-benzoylarginine-beta-naphthylamide hydrolase, aleurain, cathepsin B3, or cathepsin BA, is a lysosomal cysteine proteinase that plays a crucial role in degrading lysosomal proteins, essential for cellular homeostasis and recycling of cellular components. Cathepsin H is structured as a disulfide-linked heavy and light chain dimer, derived from a single precursor, and belongs to the peptidase C1 protein family, exhibiting both aminopeptidase and endopeptidase activities. Elevated levels of cathepsin H have been associated with malignant progression of prostate tumors, highlighting cathepsin H′s potential as a biomarker for cancer diagnosis and prognosis. Two transcript variants encoding different isoforms of cathepsin H have been identified, with both full-length and truncated forms expressed in prostate tissues and various prostate cancer cell lines, including LNCaP, PC-3, and DU-145. Cathepsin H is involved in the maturation of the biologically active surfactant protein-B peptide, further underscoring cathepsin H′s significance in both normal physiology and disease states.

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cathepsin H Antibody (F-7) References:

1. Analysis of a truncated form of cathepsin H in human prostate tumor cells.  |  Waghray, A., et al. 2002. J Biol Chem. 277: 11533-8. PMID: 11796715
2. Human cathepsin H: deletion of the mini-chain switches substrate specificity from aminopeptidase to endopeptidase.  |  Dodt, J. and Reichwein, J. 2003. Biol Chem. 384: 1327-32. PMID: 14515996
3. Processing of pulmonary surfactant protein B by napsin and cathepsin H.  |  Ueno, T., et al. 2004. J Biol Chem. 279: 16178-84. PMID: 14766755
4. Human brain cathepsin H as a neuropeptide and bradykinin metabolizing enzyme.  |  Brguljan, PM., et al. 2003. Peptides. 24: 1977-84. PMID: 15127951
5. Serum cathepsin H as a potential prognostic marker in patients with colorectal cancer.  |  Schweiger, A., et al. 2004. Int J Biol Markers. 19: 289-94. PMID: 15646835
6. Activation processing of cathepsin H impairs recognition by its propeptide.  |  Horn, M., et al. 2005. Biol Chem. 386: 941-7. PMID: 16164419
7. Characterization of a cathepsin-H-like enzyme from a human melanoma cell line.  |  Tsushima, H., et al. 1991. Int J Cancer. 48: 726-32. PMID: 2071233
8. SAR studies of o-hydroxychalcones and their cyclized analogs and study them as novel inhibitors of cathepsin B and cathepsin H.  |  Raghav, N. and Garg, S. 2014. Eur J Pharm Sci. 60: 55-63. PMID: 24780403
9. SAR studies of differently functionalized chalcones based hydrazones and their cyclized derivatives as inhibitors of mammalian cathepsin B and cathepsin H.  |  Raghav, N. and Singh, M. 2014. Bioorg Med Chem. 22: 4233-45. PMID: 24913985
10. N-formylpyrazolines and N-benzoylpyrazolines as novel inhibitors of mammalian cathepsin B and cathepsin H.  |  Raghav, N. and Garg, S. 2014. Bioorg Chem. 57: 43-50. PMID: 25181676
11. IFN-gamma increases cathepsin H mRNA levels in mouse macrophages.  |  Lafuse, WP., et al. 1995. J Leukoc Biol. 57: 663-9. PMID: 7722423
12. Lysosomal enzyme trafficking between phagosomes, endosomes, and lysosomes in J774 macrophages. Enrichment of cathepsin H in early endosomes.  |  Claus, V., et al. 1998. J Biol Chem. 273: 9842-51. PMID: 9545324

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-cathepsin H Antibody (F-7) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-cathepsin H Antibody (F-7) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

cathepsin H Antibody (F-7) Specifications

Alternate Product Name: CTSH

Product Clone Name: F-7

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 151-280

Epitope Target: cathepsin H

Epitope Species: human

Product Reactivity/Detection: cathepsin H

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for cathepsin H Antibody (F-7): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin