catalase Double Nickase Plasmid (h): sc-400353-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- catalase Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- catalase Double Nickase Plasmid (h) and catalase Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CAT. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: catalase Antibody (H-9): sc-271803
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
catalase Double Nickase Plasmid (h) | sc-400353-NIC | 20 µg | $410.00 | |||
catalase Double Nickase Plasmid (h2) | sc-400353-NIC-2 | 20 µg | $410.00 |
Human CAT encodes catalase, a peroxisomal heme enzyme that decomposes hydrogen peroxide into water and oxygen to limit oxidative damage. By controlling cellular peroxide tone, catalase supports redox homeostasis, influences lipid metabolism and peroxisome function, and modulates downstream stress-responsive signaling networks. Altered catalase activity has been linked to oxidative stress phenotypes relevant to metabolic dysfunction, neurodegeneration, inflammation, and cancer biology, where reactive oxygen species can reshape transcriptional programs and macromolecular integrity. CAT is therefore widely used as a node to interrogate antioxidant capacity, peroxisome-associated pathways, and ROS-dependent signaling.
catalase Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CAT locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CAT. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CAT function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CAT-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.