CAT Antibody (654D2Y): sc-517650

CAT Antibody (654D2Y) is a mouse monoclonal IgG2a antibody that detects the CAT protein of mouse, rat, and human origin by western blotting (WB). Anti-CAT antibody (654D2Y) is available as the non-conjugated format, providing researchers with a reliable tool for studying this important protein. Carnitine acetyltransferase (CAT, CRAT) plays a crucial role in cellular metabolism by catalyzing the reversible conversion of acetyl-CoA and carnitine to acetylcarnitine and CoA, a process vital for the regeneration of CoA, which is essential for peroxisomal β-oxidation. CAT is predominantly located in the mitochondrial matrix, where over 70% of CAT is found, but CAT is also present in the endoplasmic reticulum and the inner membrane of mitochondria. This localization is significant as CAT facilitates the transport of acetyl groups to mitochondria for oxidation, a key step in energy production. An age-associated decline in CAT activity has been observed in various rat models, highlighting CAT′s importance in metabolic health and the potential implications of reduced activity in aging. The human gene encoding CAT is located on chromosome 9q34.1 and expresses a protein that contains a peroxisomal targeting signal, predominantly found in skeletal muscle, with lesser expression in the heart, liver, and pancreas. CAT activity is influenced by metabolic substrates, being induced by acetate and fatty acids while repressed by glucose, which adds another layer of regulation to CAT′s function in energy metabolism.

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CAT Antibody (654D2Y) References:

1. Pea chloroplast carnitine acetyltransferase.  |  Masterson, C. and Wood, C. 2000. Proc Biol Sci. 267: 1-6. PMID: 10670945
2. Age-associated mitochondrial oxidative decay: improvement of carnitine acetyltransferase substrate-binding affinity and activity in brain by feeding old rats acetyl-L- carnitine and/or R-alpha -lipoic acid.  |  Liu, J., et al. 2002. Proc Natl Acad Sci U S A. 99: 1876-81. PMID: 11854488
3. Molecular cloning of cDNAs encoding human carnitine acetyltransferase and mapping of the corresponding gene to chromosome 9q34.1.  |  Corti, O., et al. 1994. Genomics. 23: 94-9. PMID: 7829107
4. The facC gene of Aspergillus nidulans encodes an acetate-inducible carnitine acetyltransferase.  |  Stemple, CJ., et al. 1998. J Bacteriol. 180: 6242-51. PMID: 9829933