Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
caspase-9 Double Nickase Plasmid (m): sc-419470-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caspase-9 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- caspase-9 Double Nickase Plasmid (m) and caspase-9 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Casp9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caspase-9 Antibody (96.1.23): sc-56076
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-9 Double Nickase Plasmid (m) | sc-419470-NIC | 20 µg | $410.00 | |||
caspase-9 Double Nickase Plasmid (m2) | sc-419470-NIC-2 | 20 µg | $410.00 |
Mouse Casp9 encodes caspase-9, an initiator cysteine-aspartate protease that is activated in the intrinsic mitochondrial apoptosis pathway following cytochrome c release and apoptosome assembly with APAF1. Activated caspase-9 cleaves effector caspases such as caspase-3 and caspase-7, coordinating programmed cell death, mitochondrial stress responses, and developmental tissue remodeling. Caspase-9 activity is modulated by BCL-2 family proteins and inhibitor of apoptosis proteins, linking it to DNA damage signaling, ER stress, and oxidative stress pathways. Dysregulated CASP9-dependent apoptosis has been implicated in cancer biology, neurodegeneration, and immune homeostasis, making Casp9 a key node for studying cell fate decisions in mouse models.
caspase-9 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Casp9 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Casp9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Casp9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Casp9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.