Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
caspase-4 p20 Double Nickase Plasmid (h): sc-400858-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caspase-4 p20 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- caspase-4 p20 Double Nickase Plasmid (h) and caspase-4 p20 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CASP4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caspase-4 Antibody (4B9): sc-56056
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-4 p20 Double Nickase Plasmid (h) | sc-400858-NIC | 20 µg | $410.00 | |||
caspase-4 p20 Double Nickase Plasmid (h2) | sc-400858-NIC-2 | 20 µg | $410.00 |
Human CASP4 encodes caspase-4, an inflammatory caspase that participates in innate immune signaling and protease cascades linked to pyroptotic cell death. Caspase-4 is directly activated by cytosolic lipopolysaccharide and promotes noncanonical inflammasome signaling, including gasdermin D cleavage and downstream cytokine maturation through inflammasome crosstalk. This pathway integrates with endoplasmic reticulum stress responses and broader inflammatory programs that shape epithelial and myeloid cell homeostasis. Dysregulated CASP4 activity has been associated with heightened inflammatory states and has been investigated in contexts such as sepsis biology, intestinal inflammation, and cancer-associated inflammation.
caspase-4 p20 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CASP4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CASP4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CASP4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CASP4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.