caspase-10 Double Nickase Plasmid (h): sc-416840-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- caspase-10 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- caspase-10 Double Nickase Plasmid (h) and caspase-10 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CASP10. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: caspase-10 p10 Antibody (E-11): sc-393983
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
caspase-10 Double Nickase Plasmid (h) | sc-416840-NIC | 20 µg | $410.00 | |||
caspase-10 Double Nickase Plasmid (h2) | sc-416840-NIC-2 | 20 µg | $410.00 |
CASP10 encodes caspase-10, an initiator cysteine-aspartate protease that is recruited to death-inducing signaling complexes downstream of extrinsic apoptosis receptors such as FAS and TNFRSF10 (TRAIL receptors). Upon activation, caspase-10 processes and amplifies apoptotic signaling through downstream effector caspases and crosstalk with mitochondrial pathways, shaping cell fate decisions during immune homeostasis and inflammatory stress. CASP10 activity intersects with NF-κB and cytokine signaling contexts where apoptotic versus non-apoptotic outcomes influence lymphocyte survival and activation. Genetic variation or dysregulation of CASP10 has been linked to immune dysregulation phenotypes and altered apoptosis sensitivity in hematologic and solid tumor biology, making it relevant for mechanistic studies of death receptor signaling.
caspase-10 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CASP10 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CASP10. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CASP10 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CASP10-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.