casein kinase IIα' Antibody (D-7): sc-514403
- casein kinase IIα' Antibody (D-7) is a mouse monoclonal IgG2b κ casein kinase IIα' antibody, cited in 8 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 327-350 at the C-terminus of casein kinase IIα′ of human origin
- casein kinase II alpha ' Antibody (D-7) is recommended for detection of casein kinase IIα′ of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-casein kinase II alpha ' Antibody (D-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for casein kinase IIα' Antibody (D-7) for WB applications. These reagents are now offered in bundles with casein kinase IIα' Antibody (D-7) (see ordering information below).
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casein kinase IIα′ Antibody (D-7) is a mouse monoclonal IgG2b kappa light chain antibody engineered to detect casein kinase IIα′ (CK2α′), also known as CSNK2A2, across multiple species including mouse, rat, and human. CK2α′ (D-7) antibody targets a precise epitope mapping between amino acids 327-350 at the C-terminus of human CK2α′, ensuring reliable performance in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CK2α′ is a vital serine/threonine protein kinase predominantly localized in the nucleus, where CK2α′ plays a crucial role in regulating DNA replication, repair, and transcriptional processes, as well as maintaining genomic stability. Nuclear presence of CK2α′ is essential for orchestrating key cellular functions that ensure proper cell cycle progression and response to DNA damage. Additionally, CK2α′ interacts with a variety of important proteins, including p53, NF-κB, and PTEN, which are integral to pathways governing cell survival, apoptosis, and proliferation. CK2α′ phosphorylates over a hundred different substrates, underscoring extensive involvement in cellular signaling and potential as a therapeutic target, particularly in cancer research where CK2α′ dysregulation is often associated with tumor growth and resistance to apoptosis. Structurally, CK2α′ forms a tetrameric complex with regulatory β subunits, which is essential for catalytic activity and substrate specificity. Anti-CK2α′ antibody (D-7) enables researchers to investigate intricate mechanisms of CK2α′-mediated signaling pathways, advancing understanding of CK2α′ role in both normal cellular physiology and various disease states, and supporting development of targeted therapies in biomedical research.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
casein kinase IIα' Antibody (D-7) References:
- Casein kinase I and II--multipotential serine protein kinases: structure, function, and regulation. | Tuazon, PT. and Traugh, JA. 1991. Adv Second Messenger Phosphoprotein Res. 23: 123-64. PMID: 1997039
- Isolation and characterization of human cDNA clones encoding the alpha and the alpha' subunits of casein kinase II. | Lozeman, FJ., et al. 1990. Biochemistry. 29: 8436-47. PMID: 2174700
- Casein kinase I gamma subfamily. Molecular cloning, expression, and characterization of three mammalian isoforms and complementation of defects in the Saccharomyces cerevisiae YCK genes. | Zhai, L., et al. 1995. J Biol Chem. 270: 12717-24. PMID: 7759525
- Isolation and characterization of human casein kinase I epsilon (CKI), a novel member of the CKI gene family. | Fish, KJ., et al. 1995. J Biol Chem. 270: 14875-83. PMID: 7797465
- Protein kinases. 4. Protein kinase CK2: an enzyme with multiple substrates and a puzzling regulation. | Allende, JE. and Allende, CC. 1995. FASEB J. 9: 313-23. PMID: 7896000
- Casein kinase II in signal transduction and cell cycle regulation. | Litchfield, DW. and Lüscher, B. 1993. Mol Cell Biochem. 127-128: 187-99. PMID: 7935350
- Molecular cloning, expression, and characterization of a 49-kilodalton casein kinase I isoform from rat testis. | Graves, PR., et al. 1993. J Biol Chem. 268: 6394-401. PMID: 8454611
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
casein kinase IIα' Antibody (D-7) | sc-514403 | 200 µg/ml | $322.00 | |||
casein kinase IIα' Antibody (D-7): m-IgG Fc BP-HRP Bundle | sc-531112 | 200 µg Ab; 10 µg BP | $361.00 | |||
casein kinase IIα' Antibody (D-7): m-IgGκ BP-HRP Bundle | sc-524711 | 200 µg Ab, 40 µg BP | $361.00 | |||
casein kinase IIα′ Antibody (D-7) AC | sc-514403 AC | 500 µg/ml, 25% agarose | $424.00 | |||
casein kinase IIα′ Antibody (D-7) HRP | sc-514403 HRP | 200 µg/ml | $322.00 | |||
casein kinase IIα′ Antibody (D-7) FITC | sc-514403 FITC | 200 µg/ml | $336.00 | |||
casein kinase IIα′ Antibody (D-7) PE | sc-514403 PE | 200 µg/ml | $349.00 | |||
casein kinase IIα′ Antibody (D-7) Alexa Fluor® 488 | sc-514403 AF488 | 200 µg/ml | $364.00 | |||
casein kinase IIα' Antibody (D-7) Alexa Fluor® 546 | sc-514403 AF546 | 200 µg/ml | $364.00 | |||
casein kinase IIα′ Antibody (D-7) Alexa Fluor® 594 | sc-514403 AF594 | 200 µg/ml | $364.00 | |||
casein kinase IIα′ Antibody (D-7) Alexa Fluor® 647 | sc-514403 AF647 | 200 µg/ml | $364.00 | |||
casein kinase IIα′ Antibody (D-7) Alexa Fluor® 680 | sc-514403 AF680 | 200 µg/ml | $364.00 | |||
casein kinase IIα′ Antibody (D-7) Alexa Fluor® 790 | sc-514403 AF790 | 200 µg/ml | $364.00 | |||
casein kinase IIα′ (D-7) Neutralizing Peptide | sc-514403 P | 100 µg/0.5 ml | $69.00 |