Cas-L Antibody (2B11): sc-33658

Cas-L Antibody (2B11) is a mouse monoclonal IgG1 antibody that detects Cas-L in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cas-L, also known as human enhancer of filamentation (HEF1) and neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), plays a crucial role as an adhesion docking molecule that facilitates protein-protein interactions and is integral to various signal transduction pathways. Cas-L is primarily located in the cytoplasm and is involved in the regulation of integrin and growth factor signaling pathways, which are essential for cellular processes such as growth, motility, and apoptosis. Cas-L′s presence in these pathways underscores its importance in cellular adhesion and migration, particularly in cancer progression, where Cas-L acts as a downstream effector of focal adhesion kinase (FAK) in the invasive behavior of glioblastoma cells. Additionally, Cas-L undergoes post-translational modifications, including phosphorylation, which is influenced by cell adhesion and Src kinase activity, further modulating Cas-L′s function and interactions. Cas-L′s ability to bind to FAK in neurons after ischemic events highlights its potential role in neuroprotection and neurite outgrowth, making Cas-L a significant target for research in both cancer biology and neurobiology.

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Cas-L Antibody (2B11) References:

1. Cytoskeleton-dependent tyrosine phosphorylation of the p130(Cas) family member HEF1 downstream of the G protein-coupled calcitonin receptor. Calcitonin induces the association of HEF1, paxillin, and focal adhesion kinase.  |  Zhang, Z., et al. 1999. J Biol Chem. 274: 25093-8. PMID: 10455189
2. Dimerization of the docking/adaptor protein HEF1 via a carboxy-terminal helix-loop-helix domain.  |  Law, SF., et al. 1999. Exp Cell Res. 252: 224-35. PMID: 10502414
3. Dissection of HEF1-dependent functions in motility and transcriptional regulation.  |  Fashena, SJ., et al. 2002. J Cell Sci. 115: 99-111. PMID: 11801728
4. Regulation of HEF1 expression and phosphorylation by TGF-beta 1 and cell adhesion.  |  Zheng, M. and McKeown-Longo, PJ. 2002. J Biol Chem. 277: 39599-608. PMID: 12189134
5. The Cas family docking protein, HEF1, promotes the formation of neurite-like membrane extensions.  |  Bargon, SD., et al. 2005. Biochim Biophys Acta. 1746: 143-54. PMID: 16344118
6. Cell adhesion regulates Ser/Thr phosphorylation and proteasomal degradation of HEF1.  |  Zheng, M. and McKeown-Longo, PJ. 2006. J Cell Sci. 119: 96-103. PMID: 16352661
7. Deregulation of HEF1 impairs M-phase progression by disrupting the RhoA activation cycle.  |  Dadke, D., et al. 2006. Mol Biol Cell. 17: 1204-17. PMID: 16394104
8. Phosphorylation of human enhancer of filamentation (HEF1) on serine 369 induces its proteasomal degradation.  |  Hivert, V., et al. 2009. Biochem Pharmacol. 78: 1017-25. PMID: 19539609
9. Phosphorylation of human enhancer filamentation 1 (HEF1) stimulates interaction with Polo-like kinase 1 leading to HEF1 localization to focal adhesions.  |  Lee, KH., et al. 2018. J Biol Chem. 293: 847-862. PMID: 29191835
10. Cell cycle-regulated processing of HEF1 to multiple protein forms differentially targeted to multiple subcellular compartments.  |  Law, SF., et al. 1998. Mol Cell Biol. 18: 3540-51. PMID: 9584194

The preferred Cas-L antibody is Cas-L Antibody (2G9): sc-33659.