Cas-L Antibody (14A11): sc-33657

Cas-L Antibody (14A11) is a mouse monoclonal IgG2b antibody that detects Cas-L in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Cas-L, also known as human enhancer of filamentation (HEF1) and neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), plays a crucial role in mediating protein-protein interactions as an adhesion docking molecule, which is essential for various signal transduction pathways. Cas-L is primarily located in the cytoplasm and is involved in integrin and growth factor signaling pathways that regulate critical cellular processes such as growth, motility, and apoptosis. Cas-L′s presence in the cytoplasm enables interactions with other signaling molecules, facilitating signal transmission that can influence cell behavior, particularly in cancer progression and neuronal development. Notably, Cas-L exists in two isoforms, p105 and p115, with the larger isoform resulting from Ser/Thr phosphorylation, a modification dependent on cell adhesion and Src kinase activity. Cas-L′s phosphorylation state is vital for function, as phosphorylation can influence interactions with other proteins, such as focal adhesion kinase (FAK), which is particularly relevant in the invasive behavior of glioblastoma cells. Additionally, TGFβ1 regulation of Cas-L gene expression and adhesion-dependent organization of actin highlight the intricate control mechanisms governing Cas-L activity and stability within cells. anti-Cas-L antibody (14A11) serves as an invaluable tool for researchers studying this protein′s complex roles in cellular signaling and disease implications.

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Cas-L Antibody (14A11) References:

1. The docking protein HEF1 is an apoptotic mediator at focal adhesion sites.  |  Law, SF., et al. 2000. Mol Cell Biol. 20: 5184-95. PMID: 10866674
2. Structure and function of cas-L and integrin-mediated signaling.  |  Seo, S., et al. 2006. Crit Rev Immunol. 26: 391-406. PMID: 17341185
3. Molecular basis for HEF1/NEDD9/Cas-L action as a multifunctional co-ordinator of invasion, apoptosis and cell cycle.  |  Singh, M., et al. 2007. Cell Biochem Biophys. 48: 54-72. PMID: 17703068
4. Nedd9/Hef1/Cas-L mediates the effects of environmental pollutants on cell migration and plasticity.  |  Bui, LC., et al. 2009. Oncogene. 28: 3642-51. PMID: 19648964
5. A novel large regulator RNA, B2, partially overlaps the HEF1/NEDD9/Cas-L gene.  |  Malleter, M., et al. 2010. Int J Mol Med. 25: 897-903. PMID: 20428794
6. HEF1, a novel target of Wnt signaling, promotes colonic cell migration and cancer progression.  |  Li, Y., et al. 2011. Oncogene. 30: 2633-43. PMID: 21317929
7. TP53 transcription factor for the NEDD9/HEF1/Cas-L gene: potential targets in Non-Small Cell Lung Cancer treatment.  |  Rousseau, B., et al. 2015. Sci Rep. 5: 10356. PMID: 26011298
8. HEF1 regulates differentiation through the Wnt5a/β-catenin signaling pathway in human gastric cancer.  |  Zhang, C., et al. 2019. Biochem Biophys Res Commun. 509: 201-208. PMID: 30579603
9. Wnt5a-induced docking of Plk1 on HEF1 promotes HEF1 translocation and tumorigenesis.  |  Hwang, JA., et al. 2021. Genes Genomics. 43: 567-575. PMID: 33826094
10. Involvement of p130(Cas) and p105(HEF1), a novel Cas-like docking protein, in a cytoskeleton-dependent signaling pathway initiated by ligation of integrin or antigen receptor on human B cells.  |  Manié, SN., et al. 1997. J Biol Chem. 272: 4230-6. PMID: 9020138

The preferred Cas-L antibody is Cas-L Antibody (2G9): sc-33659.