Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CAS Double Nickase Plasmid (h): sc-417159-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CAS Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CAS Double Nickase Plasmid (h) and CAS Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CSE1L. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CAS Antibody (H-2): sc-271537
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CAS Double Nickase Plasmid (h) | sc-417159-NIC | 20 µg | $410.00 | |||
CAS Double Nickase Plasmid (h2) | sc-417159-NIC-2 | 20 µg | $410.00 |
CSE1L encodes the cellular apoptosis susceptibility protein (CAS), a Ran GTPase pathway component that functions as an exportin for importin-α, helping recycle nuclear transport receptors and maintain nucleocytoplasmic trafficking fidelity. Through this role, CAS influences cell cycle progression, mitotic control, and stress-responsive programs linked to apoptosis susceptibility and genome maintenance. Altered CSE1L expression has been reported across multiple tumor types and is frequently studied in the context of proliferation, invasion, and metastatic potential, consistent with its central position in transport-dependent regulation of transcription and signaling. In biomedical research, CSE1L is used as a node to probe how nuclear import/export dynamics shape oncogenic phenotypes and cell fate decisions.
CAS Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CSE1L locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CSE1L. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CSE1L function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CSE1L-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.