CART Antibody (3E4): sc-293241

CART Antibody (3E4) is a mouse monoclonal IgG2a kappa light chain antibody that detects CART protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-CART antibody (3E4) is available as a non-conjugated isotype antibody. The CART gene encodes a neuropeptide that plays a crucial role in appetite and body weight regulation, making CART significant for understanding metabolic disorders and obesity. This neuropeptide, known as cocaine and amphetamine regulated transcript, exhibits changes in expression in response to psychostimulant drug administration, highlighting CART′s involvement in brain reward pathways. CART is localized in various regions of the central and peripheral nervous systems, where CART influences feeding behavior when administered centrally. Research has shown that CART expression in the rat hypothalamus is modulated by nutritional status, and synthetic CART peptide injection into the forebrain ventricular system can suppress food intake, indicating CART′s potential role in hypothalamic control of energy homeostasis. Additionally, CART presence in cell bodies and central terminals of vagal afferent neurons suggests involvement in brainstem mechanisms related to meal termination and satiety, underscoring CART′s importance in appetite and energy balance regulation.

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CART Antibody (3E4) References:

1. Stimulation of rat pancreatic exocrine secretion by cocaine- and amphetamine-regulated transcript peptide.  |  Cowles, RA., et al. 2001. Regul Pept. 99: 61-8. PMID: 11257316
2. The differential regulation of CART gene expression in a pituitary cell line and primary cell cultures of ovine pars tuberalis cells.  |  Barrett, P., et al. 2001. J Neuroendocrinol. 13: 347-52. PMID: 11264722
3. Fourth ventricular injection of CART peptide inhibits short-term sucrose intake in rats.  |  Zheng, H., et al. 2001. Brain Res. 896: 153-6. PMID: 11277984
4. Genes in drug abuse.  |  Kuhar, MJ., et al. 2001. Drug Alcohol Depend. 62: 157-62. PMID: 11295319
5. CART peptide-immunoreactive projection from the nucleus accumbens targets substantia nigra pars reticulata neurons in the rat.  |  Dallvechia-Adams, S., et al. 2001. J Comp Neurol. 434: 29-39. PMID: 11329127
6. Cocaine- and Amphetamine-Regulated Transcript Peptide (CART) Alleviates MK-801-Induced Schizophrenic Dementia-Like Symptoms.  |  Borkar, CD., et al. 2018. Neuroscience. 375: 94-107. PMID: 29425773
7. Cocaine‑ and amphetamine‑regulated transcript (CART) is associated with dopamine and is protective against ischemic stroke.  |  Lin, L., et al. 2018. Mol Med Rep. 18: 3298-3304. PMID: 30066844
8. Cocaine- and Amphetamine-Regulated Transcript (CART) Peptide Plays Critical Role in Psychostimulant-Induced Depression.  |  Meng, Q., et al. 2018. Biomol Ther (Seoul). 26: 425-431. PMID: 30157614
9. Cocaine and amphetamine regulated transcript (CART) signalling in the central nucleus of the amygdala modulates stress-induced alcohol seeking.  |  Walker, LC., et al. 2021. Neuropsychopharmacology. 46: 325-333. PMID: 32826981
10. Cocaine and Amphetamine Regulated Transcript (CART) Expression Changes in the Stomach Wall Affected by Experimentally Induced Gastric Ulcerations.  |  Zalecki, M., et al. 2021. Int J Mol Sci. 22: PMID: 34299057
11. Cocaine and amphetamine regulated transcript (CART) mediates sex differences in binge drinking through central taste circuits.  |  Maddern, XJ., et al. 2024. Neuropsychopharmacology. 49: 541-550. PMID: 37608219
12. Expression and role of cocaine-amphetamine regulated transcript (CART) in the proliferation of biliary epithelium.  |  Casini, A., et al. 2023. Eur J Histochem. 67: PMID: 37859350

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CART Antibody (3E4) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-CART Antibody (3E4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CART Antibody (3E4) Specifications

Alternate Product Name: CART

Product Clone Name: 3E4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-116

Epitope Target: CART

Epitope Species: human

Product Reactivity/Detection: CART

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, ELISA

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for CART Antibody (3E4): N/A