Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CARM1 CRISPR/Cas9 KO Plasmid (m): sc-425576
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CARM1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CARM1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CARM1 Antibody (D-6): sc-390656
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CARM1 CRISPR/Cas9 KO Plasmid (m) | sc-425576 | 20 µg | $397.00 |
Overview
Carm1 encodes coactivator-associated arginine methyltransferase 1 (CARM1/PRMT4), a histone and non-histone protein arginine methyltransferase that regulates chromatin accessibility and transcriptional programs. CARM1 methylates substrates such as histone H3 (e.g., H3R17/H3R26) and transcriptional regulators, coordinating RNA polymerase II–dependent gene expression, alternative splicing, and signal-responsive transcription. In mouse cells, Carm1 influences pathways linked to nuclear receptor and growth factor signaling, cell fate decisions, and epigenetic control of differentiation and proliferation. Dysregulated CARM1 activity is widely studied in contexts of aberrant transcriptional regulation, developmental defects, and oncogenic signaling, making it a relevant target for mechanistic studies of epigenetic and transcriptional networks.
CARM1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Carm1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Carm1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Carm1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CARM1 protein expression.
This CRISPR knockout system enables efficient generation of Carm1-deficient cell models for investigation of CARM1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Carm1 exon(s) critical for CARM1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Carm1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CARM1 CRISPR/Cas9 KO Plasmid (m) and CARM1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Carm1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CARM1 HDR Plasmid (m) and CARM1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Carm1 homology arms to support homology-directed repair at defined Carm1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.