CARKL Antibody (H-5): sc-376368
- CARKL Antibody (H-5) is a mouse monoclonal IgG2a κ CARKL antibody provided at 200 µg/ml
- raised against amino acids 179-478 mapping at the C-terminus of CARKL of human origin
- CARKL Antibody (H-5) is recommended for detection of CARKL of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-CARKL Antibody (H-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CARKL Antibody (H-5) for WB and IHC(P) applications. These reagents are now offered in bundles with CARKL Antibody (H-5) (see ordering information below).
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CARKL Antibody (H-5) is a mouse monoclonal IgG2a kappa light chain antibody that detects CARKL protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CARKL (H-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CARKL, also known as sedoheptulokinase (SHPK), is a 478 amino acid protein that plays a crucial role in cellular metabolism by catalyzing the ATP-dependent phosphorylation of sedoheptulose to produce sedoheptulose 7-phosphate, an important intermediate in the pentose phosphate pathway. This pathway is vital for generating NADPH and ribose-5-phosphate, which are essential for nucleotide synthesis and maintaining cellular redox balance. CARKL localization in the cytoplasm allows for efficient substrate access and product formation, ensuring that sedoheptulose 7-phosphate remains within the cell, thereby regulating metabolic flux. High CARKL expression levels are found in the kidney, pancreas, and liver, with lower levels in the heart, placenta, brain, and lung. Defects in CARKL gene are linked to cystinosis, an autosomal recessive disorder characterized by renal tubular dysfunction, excessive urination, and imbalances in phosphate and potassium levels.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CARKL Antibody (H-5) References:
- CTNS mutations in patients with cystinosis. | Anikster, Y., et al. 1999. Hum Mutat. 14: 454-8. PMID: 10571941
- The genomic region encompassing the nephropathic cystinosis gene (CTNS): complete sequencing of a 200-kb segment and discovery of a novel gene within the common cystinosis-causing deletion. | Touchman, JW., et al. 2000. Genome Res. 10: 165-73. PMID: 10673275
- The promoter of a lysosomal membrane transporter gene, CTNS, binds Sp-1, shares sequences with the promoter of an adjacent gene, CARKL, and causes cystinosis if mutated in a critical region. | Phornphutkul, C., et al. 2001. Am J Hum Genet. 69: 712-21. PMID: 11505338
- FISH diagnosis of the common 57-kb deletion in CTNS causing cystinosis. | Bendavid, C., et al. 2004. Hum Genet. 115: 510-4. PMID: 15365816
- Sedoheptulokinase deficiency due to a 57-kb deletion in cystinosis patients causes urinary accumulation of sedoheptulose: elucidation of the CARKL gene. | Wamelink, MM., et al. 2008. Hum Mutat. 29: 532-6. PMID: 18186520
- Characterization of mammalian sedoheptulokinase and mechanism of formation of erythritol in sedoheptulokinase deficiency. | Kardon, T., et al. 2008. FEBS Lett. 582: 3330-4. PMID: 18775706
- The significance of sedoheptulose 1,7-bisphosphate in the metabolism and regulation of the pentose pathway in liver. | Williams, JF., et al. 1985. Biochem Int. 11: 599-610. PMID: 4084320
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CARKL Antibody (H-5) | sc-376368 | 200 µg/ml | $316.00 | |||
CARKL Antibody (H-5): m-IgGκ BP-HRP Bundle | sc-522930 | 200 µg Ab, 40 µg BP | $354.00 | |||
CARKL Antibody (H-5): m-IgG2a BP-HRP Bundle | sc-546126 | 200 µg Ab; 10 µg BP | $354.00 | |||
CARKL Antibody (H-5) AC | sc-376368 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CARKL Antibody (H-5) HRP | sc-376368 HRP | 200 µg/ml | $316.00 | |||
CARKL Antibody (H-5) FITC | sc-376368 FITC | 200 µg/ml | $330.00 | |||
CARKL Antibody (H-5) PE | sc-376368 PE | 200 µg/ml | $343.00 | |||
CARKL Antibody (H-5) Alexa Fluor® 488 | sc-376368 AF488 | 200 µg/ml | $357.00 | |||
CARKL Antibody (H-5) Alexa Fluor® 546 | sc-376368 AF546 | 200 µg/ml | $357.00 | |||
CARKL Antibody (H-5) Alexa Fluor® 594 | sc-376368 AF594 | 200 µg/ml | $357.00 | |||
CARKL Antibody (H-5) Alexa Fluor® 647 | sc-376368 AF647 | 200 µg/ml | $357.00 | |||
CARKL Antibody (H-5) Alexa Fluor® 680 | sc-376368 AF680 | 200 µg/ml | $357.00 | |||
CARKL Antibody (H-5) Alexa Fluor® 790 | sc-376368 AF790 | 200 µg/ml | $357.00 |