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CARD 8 Antibody (2108C2a): sc-81213

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Datasheets
  • CARD 8 Antibody (2108C2a) is a mouse monoclonal IgG1 CARD 8 antibody, cited in 2 publications, provided at 100 µg/ml
  • raised against a recombinant protein corresponding to an internal region of CARD 8 of human origin
  • recommended for detection of CARD 8 of human origin by WB, IP and FCM
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CARD 8 Antibody (2108C2a). This work is in progress.

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CARD 8 Antibody (2108C2a) is a mouse monoclonal IgG1 kappa light chain antibody that detects CARD 8 protein of human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Anti-CARD 8 antibody (2108C2a) is available as a non-conjugated monoclonal isotype antibody. CARD 8 protein, also known as DACAR, NDPP1, TUCAN, or CARDINAL, is a 431 amino acid protein predominantly expressed in lung, ovary, testis, and placenta, where CARD 8 plays a crucial role in regulating cellular responses mediated by NF-κB activation. Such regulation is vital for maintaining cellular homeostasis and modulating inflammatory responses, making CARD 8 an important player in apoptosis and chronic inflammatory disorders. CARD 8 interacts with caspase-1, serving as a negative regulator of its activity, which is essential for controlling inflammatory processes. CARD 8 has been shown to interact with IKKγ and FHL-2, further highlighting CARD 8′s role in complex signaling pathways that influence cell survival and inflammation.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

CARD 8 Antibody (2108C2a) References:

  1. CARDINAL, a novel caspase recruitment domain protein, is an inhibitor of multiple NF-kappa B activation pathways.  |  Bouchier-Hayes, L., et al. 2001. J Biol Chem. 276: 44069-77. PMID: 11551959
  2. TUCAN/CARDINAL and DRAL participate in a common pathway for modulation of NF-kappaB activation.  |  Stilo, R., et al. 2002. FEBS Lett. 521: 165-9. PMID: 12067710
  3. CARDINAL roles in apoptosis and NFkappaB activation.  |  Bouchier-Hayes, L. and Martin, SJ. 2004. Vitam Horm. 67: 133-47. PMID: 15110175
  4. CARD proteins as therapeutic targets in cancer.  |  Damiano, JS. and Reed, JC. 2004. Curr Drug Targets. 5: 367-74. PMID: 15134219
  5. TUCAN/CARDINAL/CARD8 and apoptosis resistance in non-small cell lung cancer cells.  |  Checinska, A., et al. 2006. BMC Cancer. 6: 166. PMID: 16796750
  6. TUCAN (CARD8) genetic variants and inflammatory bowel disease.  |  McGovern, DP., et al. 2006. Gastroenterology. 131: 1190-6. PMID: 17030188
  7. The expression of TUCAN, an inhibitor of apoptosis protein, in patients with advanced non-small cell lung cancer treated with chemotherapy.  |  Checinska, A., et al. 2006. Anticancer Res. 26: 3819-24. PMID: 17094407
  8. Mutations in pattern recognition receptor genes modulate seroreactivity to microbial antigens in patients with inflammatory bowel disease.  |  Henckaerts, L., et al. 2007. Gut. 56: 1536-42. PMID: 17595233
  9. Deficiency of the NF-kappaB inhibitor caspase activating and recruitment domain 8 in patients with rheumatoid arthritis is associated with disease severity.  |  Fontalba, A., et al. 2007. J Immunol. 179: 4867-73. PMID: 17878386

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

CARD 8 Antibody (2108C2a)

sc-81213
100 µg/ml
$333.00

CARD 8 Antibody (2108C2a): m-IgG Fc BP-HRP Bundle

sc-551871
100 µg Ab; 10 µg BP
$354.00

What is the recommended storage condition for CARD 8 (2108C2a): sc-81213?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend storing the antibody at 4° C.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from Good for Western BlotAntibody works for Western blot with human monocyte lysate and human colon and duodenum extracts in published research -SCBT QC
Date published: 2023-09-14
Rated 5 out of 5 by from WB with WIWB with WI-38 whole cell lysate. -SCBT QC
Date published: 2015-05-18
Rated 5 out of 5 by from CleanClean, clear band in western Blot analysis of human recombinant CARD 8 fusion protein. -SCBT QC
Date published: 2015-01-15
Rated 5 out of 5 by from Strong bands in western blot using humanStrong bands in western blot using human CARD 8 transfected 293T and HeLa whole cell lysates. -SCBT QC
Date published: 2014-12-20
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CARD 8 Antibody (2108C2a) is rated 5.0 out of 5 by 4.
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