Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CARD 12 Double Nickase Plasmid (h): sc-418306-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CARD 12 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CARD 12 Double Nickase Plasmid (h) and CARD 12 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NLRC4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CARD 12 Antibody (F-3): sc-514658
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CARD 12 Double Nickase Plasmid (h) | sc-418306-NIC | 20 µg | $410.00 | |||
CARD 12 Double Nickase Plasmid (h2) | sc-418306-NIC-2 | 20 µg | $410.00 |
Human NLRC4 (also known as CARD12) encodes a cytosolic NOD-like receptor that nucleates assembly of the NLRC4 inflammasome in response to bacterial ligands sensed via NAIP proteins. Inflammasome formation promotes caspase-1 activation, maturation of IL-1β and IL-18, and induction of pyroptotic cell death, linking innate immune detection to inflammatory signaling. NLRC4 functions at the interface of pattern recognition and cytokine processing pathways that shape myeloid activation, epithelial defense, and tissue homeostasis. Dysregulated NLRC4 activity and variants affecting inflammasome control have been associated with autoinflammatory phenotypes and inflammatory tissue injury, making it a useful node for mechanistic studies of inflammasome biology.
CARD 12 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NLRC4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NLRC4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NLRC4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NLRC4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.