CARD 12 Antibody (F-3): sc-514658

CARD 12 Antibody (F-3) is a mouse monoclonal IgM antibody that detects CARD 12 protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CARD 12 antibody (F-3) is available as a non-conjugated format. CARD 12 (F-3) antibody recognizes a member of the membrane-associated guanylate kinase (MAGUK) family, which plays a crucial role in cellular signaling by acting as a molecular scaffold that facilitates multi-protein complex assembly. CARD 12 (F-3) monoclonal antibody targets a protein involved in regulating caspase-1 activation, essential for both apoptotic signaling and pro-inflammatory cytokine processing. CARD 12 is predominantly expressed in bone marrow, with lower expression levels in lymph nodes, placenta, spleen, and brain tissues, highlighting significance in immune responses and hematopoiesis. CARD 12 features a nucleotide-binding site domain specific for ATP/dATP, allowing engagement in critical interactions with other proteins, including self-association and interaction with ASC (apoptosis-associated speck-like protein containing a CARD), which promotes apoptosis and inflammatory signaling pathways. CARD 12′s ability to modulate these processes underscores potential as a target for therapeutic interventions in diseases characterized by dysregulated apoptosis and inflammation.

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CARD 12 Antibody (F-3) References:

1. Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis.  |  Geddes, BJ., et al. 2001. Biochem Biophys Res Commun. 284: 77-82. PMID: 11374873
2. PYNOD, a novel Apaf-1/CED4-like protein is an inhibitor of ASC and caspase-1.  |  Wang, Y., et al. 2004. Int Immunol. 16: 777-86. PMID: 15096476
3. Ipaf is upregulated by tumor necrosis factor-alpha in human leukemia cells.  |  Gutierrez, O., et al. 2004. FEBS Lett. 568: 79-82. PMID: 15196924
4. Multiple roles of CLAN (caspase-associated recruitment domain, leucine-rich repeat, and NAIP CIIA HET-E, and TP1-containing protein) in the mammalian innate immune response.  |  Damiano, JS., et al. 2004. J Immunol. 173: 6338-45. PMID: 15528373
5. Asc-mediated NF-kappaB activation leading to interleukin-8 production requires caspase-8 and is inhibited by CLARP.  |  Hasegawa, M., et al. 2005. J Biol Chem. 280: 15122-30. PMID: 15701651
6. Nucleotide binding to CARD12 and its role in CARD12-mediated caspase-1 activation.  |  Lu, C., et al. 2005. Biochem Biophys Res Commun. 331: 1114-9. PMID: 15882992
7. Nlrc4/Ipaf/CLAN/CARD12: more than a flagellin sensor.  |  Abdelaziz, DH., et al. 2010. Int J Biochem Cell Biol. 42: 789-91. PMID: 20067841
8. NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma.  |  Chen, H., et al. 2020. Mol Neurodegener. 15: 26. PMID: 32295623
9. NLRC4 biology in immunity and inflammation.  |  Andrade, WA. and Zamboni, DS. 2020. J Leukoc Biol. 108: 1117-1127. PMID: 32531834
10. SIRT3-mediated deacetylation of NLRC4 promotes inflammasome activation.  |  Guan, C., et al. 2021. Theranostics. 11: 3981-3995. PMID: 33664876
11. NLRC4 promotes the cGAS-STING signaling pathway by facilitating CBL-mediated K63-linked polyubiquitination of TBK1.  |  Zhang, R., et al. 2023. J Med Virol. 95: e29013. PMID: 37537877
12. Integrated NLRP3, AIM2, NLRC4, Pyrin inflammasome activation and assembly drive PANoptosis.  |  Oh, S., et al. 2023. Cell Mol Immunol. 20: 1513-1526. PMID: 38008850

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CARD 12 Antibody (F-3) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CARD 12 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CARD 12 Antibody (F-3) Specifications

Alternate Product Name: CARD 12

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids: 20-46

Epitope Target: CARD 12

Epitope Species: human

Product Reactivity/Detection: CARD 12 isoforms CLAN A, B, C and D

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CARD 12 Antibody (F-3): P: Blocking Peptide